BMRB Entry 26652

Title:
1H, 13C, and 15N assignments of the Pleckstrin Homology Domain of Akt1 bound to Calmodulin.
Deposition date:
2015-09-08
Original release date:
2015-10-22
Authors:
Agamasu, Constance; Ghanma, Ruba; Saad, Jamil
Citation:

Citation: Agamasu, Constance; Ghanma, Ruba; Saad, Jamil. "Structural and Biophysical Characterization of the Interactions between Calmodulin and the Pleckstrin Homology Domain of Akt"  J. Biol. Chem. 290, 27403-27413 (2015).
PubMed: 26391397

Assembly members:

Assembly members:
Akt_PHD, polymer, 113 residues, 13456.2528 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28

Data sets:
Data typeCount
13C chemical shifts92
15N chemical shifts85
1H chemical shifts85

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Akt PHD1

Entities:

Entity 1, Akt PHD 113 residues - 13456.2528 Da.

1   SERASNASPVALALAILEVALLYSGLUGLY
2   TRPLEUHISLYSARGGLYGLUTYRILELYS
3   THRTRPARGPROARGTYRPHELEULEULYS
4   ASNASPGLYTHRPHEILEGLYTYRLYSGLU
5   ARGPROGLNASPVALASPGLNARGGLUSER
6   PROLEUASNASNPHESERVALALAGLNCYS
7   GLNLEUMETLYSTHRGLUARGPROARGPRO
8   ASNTHRPHEILEILEARGCYSLEUGLNTRP
9   THRTHRVALILEGLUARGTHRPHEHISVAL
10   GLUTHRPROGLUGLUARGGLUGLUTRPALA
11   THRALAILEGLNTHRVALALAASPGLYLEU
12   LYSARGGLN

Samples:

15N: Akt_PHD, [U-100% 15N], 0.2 mM; H2O 95%; D2O 5%

15N-13C: Akt_PHD, [U-100% 15N], 0.2 mM; H2O 95%; D2O 5%

Akt(PHD): ionic strength: 0.450 M; pH: 6.500; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQC15NisotropicAkt(PHD)
3D HNCA15N-13CisotropicAkt(PHD)

Software:

CcpNmr_Analysis v2.4, CCPN - Data Analysis

NMR_PIPE v2014, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Data Processing

NMR spectrometers:

  • Bruker AMX 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks