BMRB Entry 26649

Title:
1H, 13C, 15N chemical shift assignments of initiation factor 1 from Pseudomonas aeruginosa
Deposition date:
2015-09-04
Original release date:
2016-08-22
Authors:
Zhang, Yonghong
Citation:

Citation: Bernal, Alejandra; Hu, Yanmei; Palmer, Stephanie; Silva, Aaron; Bullard, James; Zhang, Yonghong. "1H, 13C and 15N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa"  Biomol NMR Assign. 10, 249-252 (2016).
PubMed: 26983940

Assembly members:

Assembly members:
Initiation_Factor_IF-1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts70
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IF11

Entities:

Entity 1, IF1 80 residues - Formula weight is not available

1   METSERLYSGLUASPSERPHEGLUMETGLU
2   GLYTHRVALVALASPTHRLEUPROASNTHR
3   METPHEARGVALGLULEUGLUASNGLYHIS
4   VALVALTHRALAHISILESERGLYLYSMET
5   ARGLYSASNTYRILEARGILELEUTHRGLY
6   ASPLYSVALARGVALGLULEUTHRPROTYR
7   ASPLEUSERLYSGLYARGILETHRTYRARG
8   ALAARGLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Initiation Factor IF-1, [U-100% 13C; U-100% 15N], 1 mM; KPO4 25 mM; KCl 100 mM

sample_conditions_1: ionic strength: 0.125 M; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks