BMRB Entry 26618

Title:
Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough
Deposition date:
2015-07-30
Original release date:
2015-09-29
Authors:
Neca, Ant nio; Soares, Rui; Carepo, Marta; Pauleta, Sofia
Citation:

Citation: Neca, Ant nio; Soares, Rui; Carepo, Marta; Pauleta, Sofia. "Resonance assignment of DVU2108 that is part of the Orange Protein complex in Desulfovibrio vulgaris Hildenborough"  Biomol. NMR Assignments 10, 117-120 (2016).
PubMed: 26373427

Assembly members:

Assembly members:
Molecule_1, polymer, 119 residues, 12456.035 Da.

Natural source:

Natural source:   Common Name: Desulfovibrio vulgaris str. Hildenborough   Taxonomy ID: 882   Superkingdom: Bacteria   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Desulfovibrio vulgaris Hildenborough   Vector: pET21c

Data sets:
Data typeCount
13C chemical shifts476
15N chemical shifts114
1H chemical shifts629

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ORP1

Entities:

Entity 1, ORP 119 residues - 12456.035 Da.

1   METARGILEVALILETHRALAGLNGLYASN
2   THRLEUASPSERPROLEUASPPROARGPHE
3   GLYARGALAARGSERPHEILEVALCYSASP
4   THRGLUTHRGLYALATHRHISALAVALASP
5   VALSERALAASNMETASNLEUALAGLNGLY
6   ALAGLYILEGLNALAALAGLNMETALAALA
7   ASPALAGLYALAGLUALAVALILETHRGLY
8   HISVALGLYPROLYSALAPHETHRALALEU
9   ASNARGGLYHISILEALAVALTYRLEUCYS
10   ASPLEUALATHRPROARGGLUALALEUALA
11   ALAPHEILEGLUGLYLYSLEUARGPROALA
12   ASPTHRALAASPARGGLUGLYHISTRP

Samples:

15NapoDVU2108: Molecule_1, [U-100% 15N], 1.000 mM; sodium phosphate 20.000 mM; DTT 1.000 mM; NaCl 100.000 mM; sodium azide 0.001 mM; H2O 90%; D2O 10%

15N13CapoDVU2108: Molecule_1, [U-100% 13C; U-100% 15N], 1.0 mM; sodium phosphate 20.0 mM; NaCl 100.0 mM; DTT 1.0 mM; Sodium azide 1.0 mM; H2O 90%; D2O 10%

Temperature: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15NHSQC15NapoDVU2108isotropicTemperature
HNCO15N13CapoDVU2108isotropicTemperature
HNCACO15N13CapoDVU2108isotropicTemperature
HNHA15NapoDVU2108isotropicTemperature
CBCACONH15N13CapoDVU2108isotropicTemperature
CBCANH15N13CapoDVU2108isotropicTemperature
HBHA(CBCACO)NH15N13CapoDVU2108isotropicTemperature
(H)C(CCO)NH15N13CapoDVU2108isotropicTemperature
H(CCCO)NH15N13CapoDVU2108isotropicTemperature
(H)CCH-TOCSY15N13CapoDVU2108isotropicTemperature
13CHSQC15N13CapoDVU2108isotropicTemperature
13CNOESY15N13CapoDVU2108isotropicTemperature
15NHSQC15NapoDVU2108isotropicTemperature

Software:

CARA v1.8, Rochus Keller, rkeller@nmr.ch - Assignment

CcpNmr_Analysis v2.2, CCPN - Deposit

TopSpin v3.1, Bruker - Processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks