BMRB Entry 26600

Title:
Backbone 1H, 13C, 15N chemical shift assignments for T cell receptor N15 beta subunit
Deposition date:
2015-07-06
Original release date:
2015-07-14
Authors:
Mallis, Robert; Arthanari, Haribabu; Reinherz, Ellis; Wagner, Gerhard
Citation:

Citation: Mallis, Robert; Reinherz, Ellis; Wagner, Gerhard; Arthanari, Haribabu. "Backbone Resonance Assignment of N15, N30 and D10 T cell Receptor beta subunits"  Biomol. NMR Assign. 10, 35-39 (2016).
PubMed: 26275917

Assembly members:

Assembly members:
N15_beta_TCR, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11d

Data sets:
Data typeCount
13C chemical shifts580
15N chemical shifts198
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta subunit1

Entities:

Entity 1, beta subunit 239 residues - Formula weight is not available

1   ASPSERGLYVALVALGLNSERPROARGHIS
2   ILEILELYSGLULYSGLYGLYARGSERVAL
3   LEUTHRCYSILEPROILESERGLYHISSER
4   ASNVALVALTRPTYRGLNGLNTHRLEUGLY
5   LYSGLULEULYSPHELEUILEGLNHISTYR
6   GLULYSVALGLUARGASPLYSGLYPHELEU
7   PROSERARGPHESERVALGLNGLNPHEASP
8   ASPTYRHISSERGLUMETASNMETSERALA
9   LEUGLULEUGLUASPSERALAMETTYRPHE
10   CYSALASERSERLEUARGTRPGLYASPGLU
11   GLNTYRPHEGLYPROGLYTHRARGLEUTHR
12   VALLEUGLUASPLEUARGASNVALTHRPRO
13   PROLYSVALSERLEUPHEGLUPROSERLYS
14   ALAGLUILEALAASNLYSGLNLYSALATHR
15   LEUVALCYSLEUALAARGGLYPHEPHEPRO
16   ASPHISVALGLULEUSERTRPTRPVALASN
17   GLYLYSGLUVALHISSERGLYVALSERTHR
18   ASPPROGLNALATYRLYSGLUSERASNTYR
19   SERTYRSERLEUSERSERARGLEUARGVAL
20   SERALATHRPHETRPHISASNPROARGASN
21   HISPHEARGCYSGLNVALGLNPHEHISGLY
22   LEUSERGLUGLUASPLYSTRPPROGLUGLY
23   SERPROLYSPROVALTHRGLNASNILESER
24   ALAGLUALATRPGLYARGALAASPSER

Samples:

sample_1: N15 beta TCR, [U-13C; U-15N; U-2H], 0.4 mM; sodium chloride 150 mM; sodium phosphate 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 250 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CARA v1.90, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz
  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks