BMRB Entry 26591

Title:
NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa
Deposition date:
2015-06-22
Original release date:
2015-12-18
Authors:
Kaur, Kawaljit; De Guzman, Roberto
Citation:

Citation: Chaudhury, Sukanya; Nordhues, Bryce; Kaur, Kawaljit; Zhang, Na; De Guzman, Roberto. "The LcrG Tip Chaperone Protein of the Yersinia pestis Type III Secretion System Is Partially Folded"  J. Mol. Biol. 427, 3096-3109 (2015).
PubMed: 26259880

Assembly members:

Assembly members:
PcrG9-76, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 208964   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts75
1H chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PcrG9-761

Entities:

Entity 1, PcrG9-76 76 residues - Formula weight is not available

Residues 77-84 corresponds to cloning artifact

1   GLUASPTHRLEUARGALATHRVALGLNALA
2   ALAGLULEUALAILEARGASPSERGLUGLU
3   ARGGLYARGLEULEUALAGLUMETTRPGLN
4   GLYLEUGLYLEUALAALAASPALAGLYGLU
5   LEULEUPHEGLNALAPROGLUARGGLULEU
6   ALAARGALAALAGLUGLUGLULEULEUALA
7   GLULEUARGARGMETARGSERSERGLYSER
8   GLUASNLEUTYRPHEGLN

Samples:

sample_1: PcrG, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride mM; sodium phosphate mM; D2O 10 mM; H2O 90 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks