BMRB Entry 26586

Title:
15N, 13C and 1H backbone resonance assignments of TEM-1(M68L-M69T)
Deposition date:
2015-06-09
Original release date:
2015-12-18
Authors:
Gobeil, Sophie; Gagne, Donald; Doucet, Nicolas; Pelletier, Joelle
Citation:

Citation: Gobeil, Sophie; Gagne, Donald; Doucet, Nicolas; Pelletier, Joelle. "15N, 13C and 1H backbone resonance assignments of an artificially engineered TEM-1/PSE-4 class A beta-lactamase chimera and its deconvoluted mutant"  Biomol. NMR Assign. 10, 93-99 (2016).
PubMed: 26386961

Assembly members:

Assembly members:
monomer, polymer, 263 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts248
1H chemical shifts247

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TEM-1(M68L-M69T)1

Entities:

Entity 1, TEM-1(M68L-M69T) 263 residues - Formula weight is not available

1   HISPROGLUTHRLEUVALLYSVALLYSASP
2   ALAGLUASPGLNLEUGLYALAARGVALGLY
3   TYRILEGLULEUASPLEUASNSERGLYLYS
4   ILELEUGLUSERPHEARGPROGLUGLUARG
5   PHEPROLEUTHRSERTHRPHELYSVALLEU
6   LEUCYSGLYALAVALLEUSERARGVALASP
7   ALAGLYGLNGLUGLNLEUGLYARGARGILE
8   HISTYRSERGLNASNASPLEUVALGLUTYR
9   SERPROVALTHRGLULYSHISLEUTHRASP
10   GLYMETTHRVALARGGLULEUCYSSERALA
11   ALAILETHRMETSERASPASNTHRALAALA
12   ASNLEULEULEUTHRTHRILEGLYGLYPRO
13   LYSGLULEUTHRALAPHELEUHISASNMET
14   GLYASPHISVALTHRARGLEUASPARGTRP
15   GLUPROGLULEUASNGLUALAILEPROASN
16   ASPGLUARGASPTHRTHRMETPROALAALA
17   METALATHRTHRLEUARGLYSLEULEUTHR
18   GLYGLULEULEUTHRLEUALASERARGGLN
19   GLNLEUILEASPTRPMETGLUALAASPLYS
20   VALALAGLYPROLEULEUARGSERALALEU
21   PROALAGLYTRPPHEILEALAASPLYSSER
22   GLYALAGLYGLUARGGLYSERARGGLYILE
23   ILEALAALALEUGLYPROASPGLYLYSPRO
24   SERARGILEVALVALILETYRTHRTHRGLY
25   SERGLNALATHRMETASPGLUARGASNARG
26   GLNILEALAGLUILEGLYALASERLEUILE
27   LYSHISTRP

Samples:

sample_1: imidazole 3 mM; sodium azide 0.1%; monomer, [U-13C; U-15N], 0.6 mM

sample_conditions_1: ionic strength: 3 mM; pH: 6.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView v8.0.3, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
UNP P62593
AlphaFold Q47313

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks