BMRB Entry 26579

Title:
Resonance assignment of the N-terminal domain of hLCN2-R
Deposition date:
2015-06-01
Original release date:
2016-10-04
Authors:
Coudevylle, Nicolas; Zerko, Szymon; Kozminski, Wiktor; Konrat, Robert
Citation:

Citation: Cabedo Martinez, Ana Isabel; Weinhaupl, Katharina; Lee, Wing-Kee; Wolff, Natascha; Storch, Barbara; Zerko, Szymon; Konrat, Robert; Kozminski, Wiktor; Breuker, Kathrin; Thevenod, Frank; Coudevylle, Nicolas. "Biochemical and Structural Characterization of the Interaction between the Siderocalin NGAL/LCN2 (Neutrophil Gelatinase-associated Lipocalin/Lipocalin 2) and the N-terminal Domain of Its Endocytic Receptor SLC22A17"  J. Biol. Chem. 291, 2917-2930 (2016).
PubMed: 26635366

Assembly members:

Assembly members:
hLCN2-R-NTD, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Data sets:
Data typeCount
13C chemical shifts255
15N chemical shifts88
1H chemical shifts342

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hLCN2-R-NTD1

Entities:

Entity 1, hLCN2-R-NTD 105 residues - Formula weight is not available

1   METALASERASPPROILEPHETHRLEUALA
2   PROPROLEUHISCYSHISTYRGLYALAPHE
3   PROPROASNALASERGLYTRPGLUGLNPRO
4   PROASNALASERGLYVALSERVALALASER
5   ALAALALEUALAALASERALAALASERARG
6   VALALATHRSERTHRASPPROSERCYSSER
7   GLYPHEALAPROPROASPPHEASNHISCYS
8   LEULYSASPTRPASPTYRASNGLYLEUPRO
9   VALLEUTHRTHRASNALAILEGLYGLNTRP
10   ASPLEUVALCYSASPLEUGLYTRPGLNVAL
11   ILELEUGLUGLNILE

Samples:

sample_1: hLCN2-R-NTD, [U-95% 13C; U-95% 15N], 0.5 mM; H20 95%; D20 5%

sample_conditions_1: ionic strength: 70 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
5D HN(CA)CONHsample_1isotropicsample_conditions_1
5D (HACA)CON(CA)CONHsample_1isotropicsample_conditions_1
5D (H)NCO(NCA)CONHsample_1isotropicsample_conditions_1
4D HNCACOsample_1isotropicsample_conditions_1
5D HabCabCONHsample_1isotropicsample_conditions_1
5D H(CC-tocsy)CONHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks