BMRB Entry 26546

Title:
Type III Secretion Protein
Deposition date:
2015-03-20
Original release date:
2015-05-22
Authors:
Notti, Ryan; Bhattacharya, Shibani; Stebbins, Charles
Citation:

Citation: Notti, Ryan; Bhattacharya, Shibani; Lilic, Mirjana; Stebbins, Charles. "A common assembly module in injectisome and flagellar type III secretion sorting platforms"  Nat. Commun. 6, 7125-7125 (2015).
PubMed: 25994170

Assembly members:

Assembly members:
SpaO, polymer, 162 residues, Formula weight is not available
OrgB, polymer, 33 residues, Formula weight is not available
Thioredoxin, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Salmonella enterica   Taxonomy ID: 28901   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella enterica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCDFduet

Data sets:
Data typeCount
13C chemical shifts432
15N chemical shifts151
1H chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SpaO1
2OrgB2
3Thioredoxin3

Entities:

Entity 1, SpaO 162 residues - Formula weight is not available

1   GLYPROVALASPVALGLYGLYGLYARGPRO
2   LYSMETLEUARGTRPPROLEUARGPHEVAL
3   ILEGLYSERSERASPTHRGLNARGSERLEU
4   LEUGLYARGILEGLYILEGLYASPVALLEU
5   LEUILEARGTHRSERARGALAGLUVALTYR
6   CYSTYRALALYSLYSLEUGLYHISPHEASN
7   ARGVALGLUGLYGLYILEILEVALGLUTHR
8   LEUASPILEGLNHISILEGLUGLUGLUASN
9   ASNTHRTHRGLUTHRALAGLUTHRLEUPRO
10   GLYLEUASNGLNLEUPROVALLYSLEUGLU
11   PHEVALLEUTYRARGLYSASNVALTHRLEU
12   ALAGLULEUGLUALAMETGLYGLNGLNGLN
13   LEULEUSERLEUPROTHRASNALAGLULEU
14   ASNVALGLUILEMETALAASNGLYVALLEU
15   LEUGLYASNGLYGLULEUVALGLNMETASN
16   ASPTHRLEUGLYVALGLUILEHISGLUTRP
17   LEUSER

Entity 2, OrgB 33 residues - Formula weight is not available

1   GLYPROARGSERLEULYSASNILEPROILE
2   PROSERPROLEUSERPROVALGLUGLYILE
3   LEUILELYSARGLYSTHRLEUGLUARGTYR
4   PHESERILE

Entity 3, Thioredoxin 118 residues - Formula weight is not available

1   GLYPROVALASPMETVALLYSGLNILEGLU
2   SERLYSTHRALAPHEGLNGLUALALEUASP
3   ALAALAGLYASPLYSLEUVALVALVALASP
4   PHESERALATHRTRPCYSGLYPROCYSLYS
5   METILELYSPROPHEPHEHISSERLEUSER
6   GLULYSTYRSERASNVALILEPHELEUGLU
7   VALASPVALASPASPCYSGLNASPVALALA
8   SERGLUCYSGLUVALLYSCYSMETPROTHR
9   PHEGLNPHEPHELYSLYSGLYGLNLYSVAL
10   GLYGLUPHESERGLYALAASNLYSGLULYS
11   LEUGLUALATHRILEASNGLULEUVALGLY
12   GLYSERLEUGLUVALLEUPHEGLN

Samples:

sample_1: SpaO, [U-13C; U-15N], 0.2 mM; OrgB 0.2 mM; Thioredoxin mM; Sodium Citrate 10 mM; sodium chloride 100 mM; DTT 2 mM

sample_condition_1: pH: 5.6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_condition_1
3D HNCOsample_1isotropicsample_condition_1
3D HNCACBsample_1isotropicsample_condition_1
3D HNCAsample_1isotropicsample_condition_1
3D CBCA(CO)NHsample_1isotropicsample_condition_1
3D HNCAsample_1isotropicsample_condition_1
3D HN(CO)CAsample_1isotropicsample_condition_1
3D HN(CA)COsample_1isotropicsample_condition_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.5, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks