BMRB Entry 26065

Title:
NMR solution structure of PawS Derived Peptide 21 (PDP-21)
Deposition date:
2016-07-17
Original release date:
2016-09-02
Authors:
Franke, Bastian; Jayasena, Achala; Fisher, Mark; Swedberg, Joakim; Taylor, Nicolas; Mylne, Joshua; Rosengren, K. Johan
Citation:

Citation: Franke, Bastian; Jayasena, Achala; Fisher, Mark; Swedberg, Joakim; Taylor, Nicolas; Mylne, Joshua; Rosengren, K. Johan. "Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana)"  Biopolymers 106, 806-817 (2016).
PubMed: 27352920

Assembly members:

Assembly members:
PawS_Derived_Peptide_21_(PDP-21), polymer, 13 residues, 1487.684 Da.

Natural source:

Natural source:   Common Name: eudicots   Taxonomy ID: 1525732   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Zinnia haageana

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PawS_Derived_Peptide_21_(PDP-21): GRPCYTLQSCFPD

Data sets:
Data typeCount
13C chemical shifts36
15N chemical shifts12
1H chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PawS Derived Peptide 21 (PDP-21)1

Entities:

Entity 1, PawS Derived Peptide 21 (PDP-21) 13 residues - 1487.684 Da.

This peptide is head-to-tail backbone cyclic. It has an amide bond between the N- and C-terminus.

1   GLYARGPROCYSTYRTHRLEUGLNSERCYS
2   PHEPROASP

Samples:

sample_1: PawS Derived Peptide 21 (PDP-21) 2 mg/mL; H2O 90%; D2O 10%

sample_2: PawS Derived Peptide 21 (PDP-21) 2 mg/mL; D2O 100%

sample_conditions_1: pH: 5.2; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

ccpNmr, ccpnmr - data analysis, peak picking

Cyana, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks