BMRB Entry 25956

Title:
Solution structure of oxidised RsrA and without zinc ion
Deposition date:
2016-01-15
Original release date:
2016-08-01
Authors:
Zdanowski, K.; Pecqueur, L.; Werner, J.; Potts, Jennifer; Kleanthous, C.
Citation:

Citation: Rajasekar, K.; Zdanowski, K.; Yan, J.; Hopper, J.; Francis, M.; Seepersad, C.; Sharp, C.; Pecqueur, L.; Werner, J.; Robinson, C.; Mohammed, S.; Potts, Jennifer; Kleanthous, C.. "The anti-sigma factor RsrA responds to oxidative stress by reburying its hydrophobic core"  Nat. Commun. 7, 12194-12194 (2016).
PubMed: 27432510

Assembly members:

Assembly members:
ANTI-SIGMA_FACTOR_RSRA, polymer, 108 residues, 11807.9444 Da.

Natural source:

Natural source:   Common Name: Streptomyces coelicolor   Taxonomy ID: 1902   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces coelicolor

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts92
1H chemical shifts681

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ANTI-SIGMA FACTOR RSRA1

Entities:

Entity 1, ANTI-SIGMA FACTOR RSRA 108 residues - 11807.9444 Da.

1   GLYSERHISMETSERALAGLYGLUPROHIS
2   GLUTHRASPCYSSERGLUILELEUASPHIS
3   LEUTYRGLUPHELEUASPLYSGLUMETPRO
4   ASPSERASPALAVALLYSPHEGLUHISHIS
5   PHEGLUGLUSERSERPROCYSLEUGLULYS
6   TYRGLYLEUGLUGLNALAVALLYSLYSLEU
7   VALLYSARGALAALAGLYGLNASPASPVAL
8   PROGLYASPLEUARGALALYSVALMETGLY
9   ARGLEUASPLEUILEARGSERGLYGLNSER
10   VALPROGLUHISASPVALALAALAALAPRO
11   SERSERSERALAPROGLNGLUSER

Samples:

sample_1: ANTI-SIGMA FACTOR RSRA, [U-13C; U-15N], 1.25 mM

sample_conditions_1: ionic strength: 0.0 mM; pH: 7.100; pressure: 1 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1solutionsample_conditions_1
TOCSY (2D and 3D) HNCAsample_1solutionsample_conditions_1
CBCANH and CBCA(CO)NHsample_1solutionsample_conditions_1
HNCO and HN(CA)COsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
CBCANHsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HN(CA)COsample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP Q9RL96 RSRA_STRCO
AlphaFold Q9RL96

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks