BMRB Entry 25948

Title:
Ex-4[1-16]/pl14a
Deposition date:
2016-01-11
Original release date:
2016-05-23
Authors:
Schroeder, Christina; Swedberg, Joakim; Craik, David
Citation:

Citation: Schroeder, Christina; Swedberg, Joakim; Mitchell, Justin; Fairlie, David; Price, David; Liras, Spiros; Craik, David. "GLP-1 and exendin-4 alpha-conotoxin pl14a chimeras as potent GLP1-R agonists"  .

Assembly members:

Assembly members:
entity, polymer, 29 residues, 3163.514 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: HGEGTFTSDCSKQCEEGIGH KYPFCHCRX

Data sets:
Data typeCount
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 29 residues - 3163.514 Da.

1   HISGLYGLUGLYTHRPHETHRSERASPCYS
2   SERLYSGLNCYSGLUGLUGLYILEGLYHIS
3   LYSTYRPROPHECYSHISCYSARGNH2

Samples:

sample_1: entity 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 3.6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis

TOPSPIN, Bruker Biospin - collection, processing

XEASY, Brunger - chemical shift assignment, chemical shift calculation, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz