BMRB Entry 25946

Title:
Structural insights into interaction of KYE28 and lipopolysachharide
Deposition date:
2016-01-11
Original release date:
2016-05-23
Authors:
Bhunia, Anirban; Malmsten, Martin; Datta, Aritreyee
Citation:

Citation: Datta, Anirban; Malmsten, Martin; Datta, Aritreyee. "Role of Aromatic Amino Acids in Lipopolysaccharide and Membrane Interactions of Antimicrobial Peptides for Use in Plant Disease Control"  J. Biol. Chem. 291, 13301-13317 (2016).
PubMed: 27137928

Assembly members:

Assembly members:
entity, polymer, 28 residues, 3604.278 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: KYEITTIHNLFRKLTHRLFR RNFGYTLR

Data sets:
Data typeCount
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 28 residues - 3604.278 Da.

1   LYSTYRGLUILETHRTHRILEHISASNLEU
2   PHEARGLYSLEUTHRHISARGLEUPHEARG
3   ARGASNPHEGLYTYRTHRLEUARG

Samples:

sample_1: entity 1 mM; TSP 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Ascend TM 700 MHz