BMRB Entry 25941

Title:
NMR Structure of retro-KR-12: A reversed sequence of a minimalized domain derived from human cathelicidin LL-37
Deposition date:
2016-01-05
Original release date:
2017-01-12
Authors:
Gunasekera, Sunithi; Goransson, Ulf
Citation:

Citation: Gunasekera, Sunithi; Muhammad, Taj; Stromstedt, Adam; Rosengren, K.; Goransson, Ulf. "Backbone-cyclized stable peptide-dimers derived from the human cathelicidin LL-37 mediate potent antimicrobial activity"  .

Assembly members:

Assembly members:
entity, polymer, 12 residues, 1576.967 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: RLFDKIRQVIRK

Data sets:
Data typeCount
1H chemical shifts99

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 12 residues - 1576.967 Da.

1   ARGLEUPHEASPLYSILEARGGLNVALILE
2   ARGLYS

Samples:

sample_1: entity 1 mM; H2O 90 % v/v; D2O 10 % v/v

sample_2: entity 1 mM; D2O 100 % v/v

sample_conditions_1: pH: 4.5; temperature: 298 K

sample_conditions_2: pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz