BMRB Entry 25933

Title:
NSD1-PHD_5-C5HCH tandem domain structure
Deposition date:
2015-12-22
Original release date:
2016-02-23
Authors:
Berardi, Andrea; Quilici, Giacomo; Spiliotopoulos, Dimitrios; Musco, Giovanna
Citation:

Citation: Berardi, Andrea; Quilici, Giacomo; Spiliotopoulos, Dimitrios; Corral-Rodriguez, Maria Angeles; Martin, Fernando; Degano, Massimo; Tonon, Giovanni; Musco, Giovanna. "Structural basis for PHDVC5HCHNSD1-C2HRNizp1 interaction: implications for Sotos syndrome"  Nucleic Acids Res. 44, 3448-3463 (2016).
PubMed: 26896805

Assembly members:

Assembly members:
entity_1, polymer, 94 residues, 10480.064 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts93
1H chemical shifts579

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION_12
3ZINC ION_22
4ZINC ION_32
5ZINC ION_42

Entities:

Entity 1, entity_1 94 residues - 10480.064 Da.

1   GLYALAMETGLUARGGLUASPGLUCYSPHE
2   SERCYSGLYASPALAGLYGLNLEUVALSER
3   CYSLYSLYSPROGLYCYSPROLYSVALTYR
4   HISALAASPCYSLEUASNLEUTHRLYSARG
5   PROALAGLYLYSTRPGLUCYSPROTRPHIS
6   GLNCYSASPVALCYSGLYLYSGLUALAALA
7   SERPHECYSGLUMETCYSPROSERSERPHE
8   CYSLYSGLNHISARGGLUGLYMETLEUPHE
9   ILESERLYSLEUASPGLYARGLEUSERCYS
10   THRGLUHISASP

Entity 2, ZINC ION_1 - 65.409 Da.

1   ZN

Samples:

sample_1: entity_1 0.3 mM; H2O 90%; D2O 10%

sample_4: entity_1, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 90%; D2O 10%

sample_3: entity_1, [U-99% 15N], 0.3 mM; H2O 90%; D2O 10%

sample_2: entity_1 0.3 mM; D2O 100%

sample_5: entity_1, [U-99% 13C; U-99% 15N], 0.3 mM; D2O 100%

sample_conditions_1: ionic strength: 0.150 M; pH: 6.3; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_5isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_4isotropicsample_conditions_1
3D C(CO)NHsample_4isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HNCAsample_4isotropicsample_conditions_1
3D H(CCO)NHsample_4isotropicsample_conditions_1
3D HCCH-TOCSYsample_5isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_5isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks