BMRB Entry 25880

Title:
Backbone assignment of N-terminal fragment of F2-TnC from Lethocerus
Deposition date:
2015-11-05
Original release date:
2016-01-29
Authors:
Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda; Adrover, Miguel
Citation:

Citation: Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda; Adrover, Miguel. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus"  J. Biol. Chem. 291, 16090-16099 (2016).
PubMed: 27226601

Assembly members:

Assembly members:
Apo_N-term, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lethocerus Indicus   Taxonomy ID: 212017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lethocerus Indicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminus F2 TnC1

Entities:

Entity 1, N-terminus F2 TnC 88 residues - Formula weight is not available

1   METVALALAMETASPASPLEUASPLYSASP
2   GLNILEALALEULEULYSARGALAPHEGLU
3   ALAPHEALAGLNGLULYSSERTYRILEGLU
4   ALAASNMETVALGLYTHRILELEUGLNMET
5   LEUGLYHISGLUVALSERASPLYSGLNLEU
6   THRGLUILEILEVALGLUVALASPALAASP
7   GLYSERGLYGLNLEUGLUPHEGLUGLUPHE
8   VALTHRLEUALAALAARGPHELEUTHRGLU
9   ASPASNGLUGLUASPSERALAALA

Samples:

sample_1: Apo N-term, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; TRIS, [U-2H], 20 mM; KCl 100 mM; DTT, [U-2H], 2 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks