BMRB Entry 25875

Title:
Backbone assignment of Calcium loaded C-terminus of Troponin C isoform 2
Deposition date:
2015-11-02
Original release date:
2016-01-29
Authors:
Sanfelice, Domenico; Pastore, Annalisa; Adrover, Miguel
Citation:

Citation: Sanfelice, Domenico; Pastore, Annalisa; Bullard, Belinda. "Towards Understanding the Molecular Bases of Stretch Activation: A Structural comparison of the Two Troponin C Isoforms of Lethocerus"  J. Biol. Chem. 291, 16090-16099 (2016).
PubMed: 27226601

Assembly members:

Assembly members:
Cterm_TnC_F2, polymer, 71 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lethocerus Indicus   Taxonomy ID: 212017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lethocerus Indicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts205
15N chemical shifts66
1H chemical shifts356

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminus F2 TnC1

Entities:

Entity 1, C-terminus F2 TnC 71 residues - Formula weight is not available

seq starts at 88

1   GLYMETMETGLUGLULEUARGGLUALAPHE
2   ARGLEUTYRASPLYSASPGLYASNGLYTYR
3   ILETHRTHRGLUVALLEUARGGLUILELEU
4   LYSGLULEUASPASPLYSILETHRPROGLU
5   ASPLEUASPMETMETILEGLNGLUILEASP
6   SERASPGLYSERGLYTHRVALASPPHEASP
7   GLUPHEMETGLUVALMETILEGLYGLYASP
8   GLU

Samples:

sample_1: Cterm TnC F2, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; D2O, [U-2H], 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

XEASY v1.2, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks