BMRB Entry 25772

Title:
1H assignment of Elafin at 288K
Deposition date:
2015-08-26
Original release date:
2016-03-03
Authors:
Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine
Citation:

Citation: Loth, Karine; Abou Ibrahim Alami, Soha; Habes, Chahrazed; Zani, Marie-Louise; Delmas, Agnes; Moreau, Thierry; Landon, Celine. "Complete assignment of 15N, 13C Trappin-2 and 1H assignment of its two domains, Elafin and Cementoin."  Biomol. NMR Assign. 10, 223-226 (2016).
PubMed: 26878852

Assembly members:

Assembly members:
Elafin, polymer, 57 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPIC9

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts388

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Elafin1

Entities:

Entity 1, Elafin 57 residues - Formula weight is not available

1   ALAGLNGLUPROVALLYSGLYPROVALSER
2   THRLYSPROGLYSERCYSPROILEILELEU
3   ILEARGCYSALAMETLEUASNPROPROASN
4   ARGCYSLEULYSASPTHRASPCYSPROGLY
5   ILELYSLYSCYSCYSGLUGLYSERCYSGLY
6   METALACYSPHEVALPROGLN

Samples:

sample_1: Elafin 0.08 mM; sodium phosphate 25 mM; sodium chloride 300 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 300 mM; pH: 6.1; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

Ccpnmr, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz