BMRB Entry 25754

Title:
Astexin1
Deposition date:
2015-08-13
Original release date:
2015-11-02
Authors:
Link, A. James; Maksimov, Mikhail
Citation:

Citation: Maksimov, Mikhail; Koos, Joseph; Zong, Chuhan; Link, A. James; Lisko, Bozhena. "Elucidating the Specificity Determinants of the AtxE2 Lasso Peptide Isopeptidase"  J. Biol. Chem. 290, 30806-30812 (2015).
PubMed: 26534965

Assembly members:

Assembly members:
LASSO_PEPTIDE_ASTEXIN1, polymer, 23 residues, 2583.721 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 573065   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Asticcacaulis excentricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pASK75

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LASSO_PEPTIDE_ASTEXIN1: GLSQGVEPDIGQTYFEESRI NQD

Data sets:
Data typeCount
13C chemical shifts73
1H chemical shifts154

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LASSO peptide Astexin11

Entities:

Entity 1, LASSO peptide Astexin1 23 residues - 2583.721 Da.

1   GLYLEUSERGLNGLYVALGLUPROASPILE
2   GLYGLNTHRTYRPHEGLUGLUSERARGILE
3   ASNGLNASP

Samples:

sample_1: astexin1 7.27 ± 1.16 mg/mL; H2O 95%; D2O 5%

sample_conditions_1: pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

Mestrenova v9.0.0, Mestrelab Research - data analysis

GROMACS v4.6.5, Hess, Kutzner, van der Spoel and Lindahl - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz