BMRB Entry 25699

Title:
Structure of an N-terminal membrane-anchoring region of the glycosyltransferase WaaG
Deposition date:
2015-07-13
Original release date:
2015-08-17
Authors:
Liebau, Jobst; Pettersson, Pontus; Szpryngiel, Scarlett; Maler, Lena
Citation:

Citation: Liebau, Jobst; Pettersson, Pontus; Szpryngiel, Scarlett; Maler, Lena. "Membrane Interaction of the Glycosyltransferase WaaG"  Biophys. J. 109, 552-563 (2015).
PubMed: 26244737

Assembly members:

Assembly members:
entity, polymer, 30 residues, 3680.196 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: YAEKVAQEKGFLYRLTSRYR HYAAFERATF

Data sets:
Data typeCount
13C chemical shifts84
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 30 residues - 3680.196 Da.

1   TYRALAGLULYSVALALAGLNGLULYSGLY
2   PHELEUTYRARGLEUTHRSERARGTYRARG
3   HISTYRALAALAPHEGLUARGALATHRPHE

Samples:

sample_1: entity 0.6 mM; DPC, [U-100% 2H], 50 mM; H2O 90%; D2O 10%; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAE77661 BAJ45372 BAL40224
EMBL CDJ73243 CDP74575 CDP74638 CDU41766 CDY63068
GB AAA03743 AAA24082 AAB18608 AAC76655 ACB04681
REF NP_418088 WP_000634279 WP_000634283 WP_000634284 WP_000634289
SP P25740
AlphaFold P25740