BMRB Entry 25572

Title:
Dad2 C terminal
Deposition date:
2015-04-15
Original release date:
2015-05-15
Authors:
Singh, Himanshu
Citation:

Citation: Singh, Himanshu. "Dad2 C terminal"  Biochemistry ., .-..

Assembly members:

Assembly members:
Dad2_C_terminal, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Nematodes   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts49
1H chemical shifts50

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 139 residues - Formula weight is not available

1   METALASERLYSTHRASNTHRALAILETYR
2   GLNLYSILEALAGLULYSARGALAASNLEU
3   GLUARGPHEARGGLUPHELYSGLULEUTHR
4   ASPASPLEUVALLEUGLNLEUGLUSERILE
5   GLYASPLYSLEUGLUTHRMETASNGLYGLY
6   THRALASERVALALALEUILELEUALAASN
7   TRPLYSSERVALVALGLNSERILESERLEU
8   ALASERLEUALALEUMETLYSGLUSERASN
9   ASPASNASNLYSGLUALAPHEPROGLUPRO
10   LEUVALARGVALARGVALGLYGLNSERASN
11   GLUGLUASNGLNASPGLUGLUGLUALAASP
12   GLUGLUGLUGLYVALARGASPSERGLUGLU
13   VALGLUGLUSERTHRGLULYSLEUALAALA
14   ALALEUGLUHISHISHISHISHISHIS

Samples:

sample_1: entity, [U-100% 15N], .4 mM; entity, [U-100% 13C], .2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

cara, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

EMBL CAX43751
GB EAK95870 EEQ45932 KGQ89068 KGQ96391 KGR00135
REF XP_002418450 XP_714910
SP Q59ZG8
AlphaFold Q59ZG8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks