BMRB Entry 25540

Title:
NMR structure of the complex between the C-terminal domain of the Rift Valley fever virus protein NSs and the PH domain of the Tfb1 subunit of TFIIH.
Deposition date:
2015-03-18
Original release date:
2015-04-20
Authors:
Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane; Guendel, Irene; Chabot, Philippe; Kehn-Hall, Kylene; Omichinski, James
Citation:

Citation: Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane; Guendel, Irene; Chabot, Philippe; Kehn-Hall, Kylene; Omichinski, James. "A OmegaXaV motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence."  Proc. Natl. Acad. Sci. U.S.A. 112, 6021-6026 (2015).
PubMed: 25918396

Assembly members:

Assembly members:
RNA_polymerase_II_transcription_factor_B_subunit_1, polymer, 115 residues, 12903.807 Da.
Non-structural_protein_NS-S, polymer, 24 residues, 2566.504 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukarota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_polymerase_II_transcription_factor_B_subunit_1: PSHSGAAIFEKVSGIIAINE DVSPAELTWRSTDGDKVHTV VLSTIDKLQATPASSEKMML RLIGKVDESKKRKDNEGNEV VPKPQRHMFSFNNRTVMDNI KMTLQQIISRYKDAD
Non-structural_protein_NS-S: GGGGYDVEMESEEESDDDGF VEVD

Data sets:
Data typeCount
13C chemical shifts597
15N chemical shifts143
1H chemical shifts953

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA polymerase II transcription factor B subunit 11
2Non-structural protein NS-S2

Entities:

Entity 1, RNA polymerase II transcription factor B subunit 1 115 residues - 12903.807 Da.

1   PROSERHISSERGLYALAALAILEPHEGLU
2   LYSVALSERGLYILEILEALAILEASNGLU
3   ASPVALSERPROALAGLULEUTHRTRPARG
4   SERTHRASPGLYASPLYSVALHISTHRVAL
5   VALLEUSERTHRILEASPLYSLEUGLNALA
6   THRPROALASERSERGLULYSMETMETLEU
7   ARGLEUILEGLYLYSVALASPGLUSERLYS
8   LYSARGLYSASPASNGLUGLYASNGLUVAL
9   VALPROLYSPROGLNARGHISMETPHESER
10   PHEASNASNARGTHRVALMETASPASNILE
11   LYSMETTHRLEUGLNGLNILEILESERARG
12   TYRLYSASPALAASP

Entity 2, Non-structural protein NS-S 24 residues - 2566.504 Da.

Residues 242-246 were introduced to faciliate the purification and quantification of the protein.

1   GLYGLYGLYGLYTYRASPVALGLUMETGLU
2   SERGLUGLUGLUSERASPASPASPGLYPHE
3   VALGLUVALASP

Samples:

sample_1: RNA polymerase II transcription factor B subunit 1, [U-13C; U-15N], 1 mM; Non-structural protein NS-S 2 mM; H2O 90%; D2O 10%

sample_2: RNA polymerase II transcription factor B subunit 1, [U-13C; U-15N], 1 mM; Non-structural protein NS-S 2 mM; D2O 100%

sample_3: RNA polymerase II transcription factor B subunit 1, [U-15N], 0.5 mM; Non-structural protein NS-S 1 mM; H2O 90%; D2O 10%

sample_4: RNA polymerase II transcription factor B subunit 1 1 mM; Non-structural protein NS-S, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%

sample_5: RNA polymerase II transcription factor B subunit 1 0.5 mM; Non-structural protein NS-S, [U-13C; U-15N], 1 mM; D2O 100%

sample_6: RNA polymerase II transcription factor B subunit 1 0.4 mM; Non-structural protein NS-S, [U-15N], 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_6isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_4isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_4isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_5isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_5isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_5isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_5isotropicsample_conditions_1
3D 1H-13C NOESY intermolecularsample_2isotropicsample_conditions_1
3D 1H-13C NOESY intermolecularsample_5isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.3.1 -

CNS -

TALOS-N -

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

SP P32776 P32776 P21698
REF NP_010597 NP_010597 YP_003848706
BMRB 6225 18229 18842 19791
PDB
DBJ GAA22531
EMBL CAY78811
GB AAA35143 AAB64747 AAU09707 AHY75284 AJP38011
TPG DAA12150
AlphaFold E9P948 A2SZX2 P32776

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks