BMRB Entry 25467

Title:
Backbone H, N and C assignments for PLAT domain of human polycystin-1, W3128A mutant
Deposition date:
2015-02-05
Original release date:
2016-06-30
Authors:
Williamson, Mike; Ong, Albert; Xu, Yaoxian; Hounslow, Andrea
Citation:

Citation: Xu, Yaoxian; Ong, Albert; Williamson, Mike; Hounslow, Andrea. "Backbone assignment and secondary structure of the PLAT domain of human polycystin-1"  Biomol. NMR Assign. 9, 369-373 (2015).
PubMed: 25943267

Assembly members:

Assembly members:
PKD1_PLAT, polymer, 210 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: GEVS2

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts109
1H chemical shifts109

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PKD1_PLAT1

Entities:

Entity 1, PKD1_PLAT 210 residues - Formula weight is not available

This has a polyalanine tag followed by the GB1 domain, a short linker, residues 3112-3245 of human PKD1 PLAT, and a His tag.

1   ALAALAALAALAALAALAALAALAMETGLN
2   TYRLYSLEUALALEUASNGLYLYSTHRLEU
3   LYSGLYGLUTHRTHRTHRGLUALAVALASP
4   ALAALATHRALAGLULYSVALPHELYSGLN
5   TYRALAASNASPASNGLYVALASPGLYGLU
6   TRPTHRTYRASPASPALATHRLYSTHRPHE
7   THRVALTHRGLULEUVALPROARGGLYSER
8   CYSGLYGLNARGGLYARGPHELYSTYRGLU
9   ILELEUVALLYSTHRGLYALAGLYARGGLY
10   SERGLYTHRTHRALAHISVALGLYILEMET
11   LEUTYRGLYVALASPSERARGSERGLYHIS
12   ARGHISLEUASPGLYASPARGALAPHEHIS
13   ARGASNSERLEUASPILEPHEARGILEALA
14   THRPROHISSERLEUGLYSERVALTRPLYS
15   ILEARGVALTRPHISASPASNLYSGLYLEU
16   SERPROALATRPPHELEUGLNHISVALILE
17   VALARGASPLEUGLNTHRALAARGSERALA
18   PHEPHELEUVALASNASPTRPLEUSERVAL
19   GLUTHRGLUALAASNGLYGLYLEUVALGLU
20   LYSGLUVALLEUALAALASERASPALAALA
21   LEULEUARGPHEHISHISHISHISHISHIS

Samples:

sample_1: GB1-PLAT, [U-13C; U-15N; U-2H], 200 uM; DTT 10 mM; sodium chloride 500 mM; TRIS 50 mM

sample_conditions_1: ionic strength: 550 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

Felix, Accelrys Software Inc. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 600 MHz

Related Database Links:

Uniprot P98161
AlphaFold Q15141

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks