BMRB Entry 25458

Title:
NMR structure of an Odin-Sam1 fragment
Deposition date:
2015-01-30
Original release date:
2015-07-13
Authors:
Mercurio, Flavia; Leone, Marilisa
Citation:

Citation: Mercurio, Flavia; Natale, Concetta; Pirone, Luciano; Scognamiglio, Pasqualina; Marasco, Daniela; Pedone, Emilia; Saviano, Michele; Leone, Marilisa. "Peptide Fragments of Odin-Sam1: Conformational Analysis and Interaction Studies with EphA2-Sam"  Chembiochem 16, 1629-1636 (2015).
PubMed: 26120079

Assembly members:

Assembly members:
entity_1, polymer, 45 residues, 4933.5 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XSKLLLNGFDDVHFLGSNVM EEQDLRDIGISDPQHRRKLL QAARX

Data sets:
Data typeCount
1H chemical shifts315

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 45 residues - 4933.5 Da.

The peptide corresponds to a fragment of human Odin (Uniprot code Q92625, residues 715-757) with N-terminal Acetylation and C-terminal amidation

1   ACESERLYSLEULEULEUASNGLYPHEASP
2   ASPVALHISPHELEUGLYSERASNVALMET
3   GLUGLUGLNASPLEUARGASPILEGLYILE
4   SERASPPROGLNHISARGARGLYSLEULEU
5   GLNALAALAARGNH2

Samples:

sample_1: entity_1 600 uM; H2O 30 % v/v; 2,2,2-trifluoroethanol-d3 (TFE-d3), [U-99% 2H], 70 % v/v

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

VNMRJ, Varian - collection, processing

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

EMBL Q92625