BMRB Entry 25446

Title:
solution Structure of KstB-PCP in kosinostatin biosynthesis
Deposition date:
2015-01-20
Original release date:
2016-01-19
Authors:
Zhao, Bin
Citation:

Citation: Zhao, Bin. "1H and 15N Assigned Chemical Shifts for KstB-PCP"  .

Assembly members:

Assembly members:
KstB-PCP, polymer, 89 residues, 9699.999 Da.
entity_PNS, non-polymer, 358.348 Da.

Natural source:

Natural source:   Common Name: Micromonospora sp. TP-A0468   Taxonomy ID: 1182970   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Micromonospora Micromonospora sp. TP-A0468

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts90
1H chemical shifts606

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KstB-PCP1
2PHOSPHOPANTETHEINE2

Entities:

Entity 1, KstB-PCP 89 residues - 9699.999 Da.

1   METGLNHISALASERVALILEALAGLNPHE
2   VALVALGLUGLUPHELEUPROASPVALALA
3   PROALAASPVALASPVALASPLEUASPLEU
4   VALASPASNGLYVALILEASPSERLEUGLY
5   LEULEULYSVALILEALATRPLEUGLUASP
6   ARGPHEGLYILEALAALAASPASPVALGLU
7   LEUSERPROGLUHISPHEARGSERILEARG
8   SERILEASPALAPHEVALVALGLYALATHR
9   THRPROPROVALGLUALALYSLEUGLN

Entity 2, PHOSPHOPANTETHEINE - C11 H23 N2 O7 P S - 358.348 Da.

1   PNS

Samples:

sample_1: KstB-PCP, [U-99% 13C; U-99% 15N], 2 mM; phosphate 80 mM; Sodium chloride 50 mM

pH: pH: 7.0

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicpH

Software:

No software information available

NMR spectrometers:

  • AGILENT INOVA 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks