BMRB Entry 25430

Title:
GADD34; PP1-binding domain
Deposition date:
2015-01-14
Original release date:
2015-06-19
Authors:
Choy, Meng Shyan; Peti, Wolfgang
Citation:

Citation: Choy, Meng Shyan; Yusoff, Permeen; Lee, Irene C.; Newton, Jocelyn C.; Goh, Catherine W.; Page, Rebecca; Shenolikar, Shirish; Peti, Wolfgang. "Structural and Functional Analysis of the GADD34:PP1 eIF2a Phosphatase"  Cell Reports 11, 1885-1891 (2015).

Assembly members:

Assembly members:
GADD34, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJexpress411

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts63
1H chemical shifts252

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GADD341

Entities:

Entity 1, GADD34 77 residues - Formula weight is not available

Residue 1 represent a non native Met from the start codon. Residue 72-77 represent non-native affinity (His) tag.

1   METLYSALAARGLYSVALARGPHESERGLU
2   LYSVALTHRVALHISPHELEUALAVALTRP
3   ALAGLYPROALAGLNALAALAARGGLNGLY
4   PROTRPGLUGLNLEUALAARGASPARGSER
5   ARGPHEALAARGARGILETHRGLNALAGLN
6   GLUGLULEUSERPROCYSLEUTHRPROALA
7   ALAARGALAARGALATRPALAARGLEUARG
8   ASNHISHISHISHISHISHIS

Samples:

sample_1: GADD34, [U-98% 13C; U-98% 15N], 1 mM; sodium phosphate 20 mM; TCEP 0.5 mM

sample_2: GADD34, [U-98% 15N], 1 mM; sodium phosphate 20 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

TOPSPIN v3.2, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks