BMRB Entry 25425

Title:
Backbone and side-chain 13C, 15N resonance assignments of artificially disordered FAS 1-4 A546T domain of TGFBIp dissolved in 95 % of DMSO in presence of water
Deposition date:
2015-01-12
Original release date:
2015-04-16
Authors:
Kulminskaya, Natalia; Yoshimura, Yuichi; Runager, Kasper; Sorensen, Charlotte; Bjerring, Morten; Mulder, Frans; Nielsen, Niels
Citation:

Citation: Kulminskaya, Natalia; Yoshimura, Yuichi; Runager, Kasper; Sorensen, Charlotte; Bjerring, Morten; Andreasen, Maria; Otzen, Daniel; Enghild, Jan; Nielsen, Niels; Mulder, Frans. "Near-complete 1H, 13C, 15N resonance assignments of dimethylsulfoxide-denatured TGFBIp FAS1-4 A546T"  Biomol. NMR Assign. 10, 25-29 (2016).
PubMed: 26275916

Assembly members:

Assembly members:
FAS1-4_domain_of_TGFBIp, polymer, 158 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ChampionTM pET SUMO Protein Expression System

Data sets:
Data typeCount
13C chemical shifts540
15N chemical shifts148
1H chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fourth fascilin domain (FAS1-4)1

Entities:

Entity 1, fourth fascilin domain (FAS1-4) 158 residues - Formula weight is not available

1   ALAGLYMETGLYTHRVALMETASPVALLEU
2   LYSGLYASPASNARGPHESERMETLEUVAL
3   ALAALAILEGLNSERALAGLYLEUTHRGLU
4   THRLEUASNARGGLUGLYVALTYRTHRVAL
5   PHEALAPROTHRASNGLUTHRPHEARGALA
6   LEUPROPROARGGLUARGSERARGLEULEU
7   GLYASPALALYSGLULEUALAASNILELEU
8   LYSTYRHISILEGLYASPGLUILELEUVAL
9   SERGLYGLYILEGLYALALEUVALARGLEU
10   LYSSERLEUGLNGLYASPLYSLEUGLUVAL
11   SERLEULYSASNASNVALVALSERVALASN
12   LYSGLUPROVALALAGLUPROASPILEMET
13   ALATHRASNGLYVALVALHISVALILETHR
14   ASNVALLEUGLNPROPROALAASNARGPRO
15   GLNGLUARGGLYASPGLULEUALAASPSER
16   ALALEUGLUILEPHELYSGLNALA

Samples:

FAS1-4: H2O 4%; DMSO 95%; TFA 1%; FAS1-4, [U-100% 13C; U-100% 15N], 3.3 mg/mL

sample_conditions_1: pH: 2.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCFAS1-4isotropicsample_conditions_1
3D HNCOFAS1-4isotropicsample_conditions_1
3D HN(CA)COFAS1-4isotropicsample_conditions_1
3D HNCOCOFAS1-4isotropicsample_conditions_1
(H)CC(CO)NHFAS1-4isotropicsample_conditions_1
(HN)CO(CO)NHFAS1-4isotropicsample_conditions_1
(H)N(COCO)NHFAS1-4isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks