BMRB Entry 25352

Title:
Acidocin B
Deposition date:
2014-11-19
Original release date:
2015-03-02
Authors:
Vederas, John; Acedo, Jeella; van Belkum, Marco; Lohans, Christopher
Citation:

Citation: Acedo, Jeella; van Belkum, Marco; Lohans, Christopher; McKay, R.; Miskolzie, M.; Vederas, John. "Solution Structure of Acidocin B, a Circular Bacteriocin Produced by Lactobacillus acidophilus M46"  Appl. Environ. Microbiol. 81, 2910-2918 (2015).
PubMed: 25681186

Assembly members:

Assembly members:
entity, polymer, 58 residues, 5642.548 Da.

Natural source:

Natural source:   Common Name: lactic acid bacteria   Taxonomy ID: 1579   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactobacillus acidophilus

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Lactobacillus acidophilus

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts377

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 58 residues - 5642.548 Da.

N- and C-termini are cyclized

1   ILETYRTRPILEALAASPGLNPHEGLYILE
2   HISLEUALATHRGLYTHRALAARGLYSLEU
3   LEUASPALAVALALASERGLYALASERLEU
4   GLYTHRALAPHEALAALAILELEUGLYVAL
5   THRLEUPROALATRPALALEUALAALAALA
6   GLYALALEUGLYALATHRALAALA

Samples:

sample_1: entity 1 mM; SDS, [U-99% 2H], 80 mM; DSS 0.5 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Agilent vNMRS 700 MHz

Related Database Links:

PDB
DBJ BAA22261 BAH08712
EMBL CAA84399
GB AJP07153
PRF 2120355B
REF WP_012621083 YP_002474143