BMRB Entry 25336

Title:
Backbone assignments for cyclophilin from Geobacillus Kaustophilus with saturating concentrations of the model peptide GSFGPDLRAGD
Deposition date:
2014-11-13
Original release date:
2014-12-16
Authors:
Holliday, Michael; Camilloni, Carlo; Armstrong, Geoffrey; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan
Citation:

Citation: Holliday, Michael; Camilloni, Carlo; Geoffrey, Armstrong; Isern, Nancy; Zhang, Fengli; Vendruscolo, Michele; Eisenmesser, Elan. "Structure and Dynamics of GeoCyp: A Thermophilic Cyclophilin with a Novel Substrate Binding Mechanism That Functions Efficiently at Low Temperatures"  Biochemistry 54, 3207-3217 (2015).
PubMed: 25923019

Assembly members:

Assembly members:
GeoCyp, polymer, 148 residues, 16116.1 Da.
GSFGPDLRAGD, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1462   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus Kaustophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ401

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts132
1H chemical shifts132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GeoCyp1
2GSFGPDLRAGD2

Entities:

Entity 1, GeoCyp 148 residues - 16116.1 Da.

Full length GeoCyp, with two additional N-terminal residues, Gly-Ser-, remaining after removal of affinity tag

1   GLYSERMETALALYSLYSGLYTYRILELEU
2   METGLUASNGLYGLYLYSILEGLUPHEGLU
3   LEUPHEPROASNGLUALAPROVALTHRVAL
4   ALAASNPHEGLULYSLEUALAASNGLUGLY
5   PHETYRASNGLYLEUTHRPHEHISARGVAL
6   ILEPROGLYPHEVALSERGLNGLYGLYCYS
7   PROARGGLYASNGLYTHRGLYASPALAGLY
8   TYRTHRILEPROCYSGLUTHRASPASNASN
9   PROHISARGHISVALTHRGLYALAMETSER
10   METALAHISARGGLYARGASPTHRGLYSER
11   CYSGLNPHEPHEILEVALHISGLUPROGLN
12   PROHISLEUASPGLYVALHISTHRVALPHE
13   GLYGLNVALTHRSERGLYMETASPVALVAL
14   ARGTHRMETLYSASNGLYASPVALMETLYS
15   GLUVALLYSVALPHEASPGLUPRO

Entity 2, GSFGPDLRAGD 11 residues - Formula weight is not available

1   GLYSERPHEGLYPROASPLEUARGALAGLY
2   ASP

Samples:

GeoCyp-GSFGPDLRAGD: GeoCyp, [U-100% 13C; U-100% 15N], 1 mM; GSFGPDLRAGD 4 mM; sodium phosphate 50 mM; EDTA 1 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCGeoCyp-GSFGPDLRAGDisotropicsample_conditions_1
3D HNCACBGeoCyp-GSFGPDLRAGDisotropicsample_conditions_1
3D HNCAGeoCyp-GSFGPDLRAGDisotropicsample_conditions_1
3D CBCA(CO)NHGeoCyp-GSFGPDLRAGDisotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.1, CCPN - chemical shift assignment, peak picking

nmrPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Varian - Spectrum processing, collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 18628
PDB
DBJ BAD76583 GAD12357 GAJ58355
GB ABO67576 ACX77066 ADI26232 ADU94680 AEV19889
REF WP_008879707 WP_011231780 WP_012820689 WP_013144839 WP_025948556

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks