BMRB Entry 25185

Title:
Backbone chemical shift assignment of FG-NUP (48-172) in buffer condition.
Deposition date:
2014-08-29
Original release date:
2015-10-08
Authors:
Dutta, Kaushik; Cowburn, David; Rout, Michael
Citation:

Citation: Hough, Loren; Dutta, Kaushik; Sparks, Sam; Temel, Deniz; Kamal, Alia; Tetenbaum-Novatt, Jaclyn; Rout, Michael; Cowburn, David. "Atomic Scale Dynamic Behavior of the Nuclear Transport Selectivity Barrier"  Science ., .-..

Assembly members:

Assembly members:
FG-N-6His, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts120
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 137 residues - Formula weight is not available

1   METGLYTHRSERALAPROASNASNTHRASN
2   ASNALAASNSERSERILETHRPROALAPHE
3   GLYSERASNASNTHRGLYASNTHRALAPHE
4   GLYASNSERASNPROTHRSERASNVALPHE
5   GLYSERASNASNSERTHRTHRASNTHRPHE
6   GLYSERASNSERALAGLYTHRSERLEUPHE
7   GLYSERSERSERALAGLNGLNTHRLYSSER
8   ASNGLYTHRALAGLYGLYASNTHRPHEGLY
9   SERSERSERLEUPHEASNASNSERTHRASN
10   SERASNTHRTHRLYSPROALAPHEGLYGLY
11   LEUASNPHEGLYGLYGLYASNASNTHRTHR
12   PROSERSERTHRGLYASNALAASNTHRSER
13   ASNASNLEUPHEGLYALATHRALASERHIS
14   METHISHISHISHISHISHIS

Samples:

sample_1: FG-N-6His, [U-100% 15N], 250 uM; HEPES 20 mM; KCl 150 mM; MgCl2 2 mM; BSA 100 mM; H2O 90%; D2O 10%

sample_2: FG-N-6His, [U-100% 13C; U-100% 15N], 250 uM; HEPES 20 mM; KCl 150 mM; MgCl2 2 mM; BSA 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 25184
DBJ GAA24320
EMBL CAA89332 CAY80739
GB AAA34821 AHY78965 AJP39666 AJR53935 AJR54261
REF NP_012494
SP P14907
TPG DAA08759
AlphaFold P14907

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks