BMRB Entry 19873

Title:
D loop of tRNA(Met)
Deposition date:
2014-03-25
Original release date:
2015-04-13
Authors:
Eveline, Lescrinier; Natala, Dyubankova; Piet, Herdewijn
Citation:

Citation: Eveline, Lescrinier; Natalia, Dyubankova; Piet, Herdewijn. "Contribution of dihydrouridine in folding of the D-arm in tRNA"  Org. Biomol. Chem. ., .-. (2015).
PubMed: 25815904

Assembly members:

Assembly members:
D_arm_tRNA(Met), polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D_arm_tRNA(Met): GGAGAGXGGAACUCCC

Data sets:
Data typeCount
13C chemical shifts94
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1D-arm of tRNA(Met)1

Entities:

Entity 1, D-arm of tRNA(Met) 16 residues - Formula weight is not available

1   GGAGAGH2UGGA
2   ACUCCC

Samples:

sample_1: D arm tRNA(Met) 1 ± 0.5 mM; D2O 100%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - data analysis

X-PLOR_NIH v3.851, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz