BMRB Entry 19821

Title:
Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx
Deposition date:
2014-02-27
Original release date:
2014-03-10
Authors:
Russo, Luigi
Citation:

Citation: Contursi, Patrizia; Farina, Biancamaria; Pirone, Luciano; Fusco, Salvatore; Russo, Luigi; Bartolucci, Simonetta; Fattorusso, Roberto; Pedone, Emilia. "Structural and functional studies of Stf76 from the Sulfolobus islandicus plasmid-virus pSSVx: a novel peculiar member of the winged helix-turn-helix transcription factor family."  Nucleic Acids Res. 42, 5993-6011 (2014).
PubMed: 24682827

Assembly members:

Assembly members:
Stf76, polymer, 77 residues, 9355.989 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 45202   Superkingdom: Plasmid   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30a(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts77
15N chemical shifts77
1H chemical shifts412

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 77 residues - 9355.989 Da.

1   LYSALALYSLEUSERLEUTHRGLNLEUILE
2   LEUILEARGLEUSERASNARGGLYCYSGLN
3   THRLEUGLUGLULEUGLUGLUPHETHRGLN
4   ALALYSARGGLUVALLEULEUVALTHRLEU
5   THRARGLEUHISGLNARGGLYVALILETYR
6   ARGLYSTRPARGHISPHESERGLYARGLYS
7   TYRARGGLUTYRCYSLEULYSHISARGASP
8   GLULEUALAASPLEUGLUHIS

Samples:

sample_1: Stf76, [U-100% 13C; U-100% 15N], 100-200 uM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.05 M; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

CARA, Keller and Wuthrich - chemical shift assignment

CS-Rosetta, Shen, Vernon, Baker and Bax - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks