BMRB Entry 19796

Title:
hinge region of human Col7
Deposition date:
2014-02-13
Original release date:
2014-05-06
Authors:
Richer, Barbara; Seeger, Karsten
Citation:

Citation: Richer, Barbara; Seeger, Karsten. "The hinge region of type VII collagen is intrinsically disordered"  Matrix Biology 36, 77-83 (2014).
PubMed: 24810542

Assembly members:

Assembly members:
hCol7_hinge, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTWIN1

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts174
15N chemical shifts61
1H chemical shifts63

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hinge monomer1

Entities:

Entity 1, hinge monomer 73 residues - Formula weight is not available

1   GLYARGALAMETGLYLEUPROGLYGLUARG
2   GLYLEUARGGLYGLUPROGLYSERVALPRO
3   ASNVALASPARGLEULEUGLUTHRALAGLY
4   ILELYSALASERALALEUARGGLUILEVAL
5   GLUTHRTRPASPGLUSERSERGLYSERPHE
6   LEUPROVALPROGLUARGARGARGGLYPRO
7   LYSGLYASPSERGLYGLUGLNGLYPROPRO
8   GLYLYSGLU

Samples:

sample_1: hCol7 hinge, [U-13C; U-15N], 0.2 mM; D2O, [U-100% 2H], 10%; H2O 90%; TSP 0.05 mM; potassium phosphate 10 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CCPN, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks