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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19726
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Daubner, Gerrit; Bruemmer, Anneke; Tocchini, Cristina; Gerhardy, Stefan; Ciosk, Rafal; Zavolan, Mihaela; Allain, Frederic. "Structural and functional implications of the QUA2 domain on RNA recognition by GLD-1" .
Assembly members:
GermLine_development_Defective_1, polymer, 142 residues, 16075.667 Da.
5'-CUACUCAUAU-3', polymer, 10 residues, 3082.892 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTYB1
Entity Sequences (FASTA):
GermLine_development_Defective_1: LPEPAGDMISITEKIYVPKN
EYPDYNFVGRILGPRGMTAK
QLEQDTGCKIMVRGKGSMRD
KSKESAHRGKANWEHLEDDL
HVLVQCEDTENRVHIKLQAA
LEQVKKLLIPAPEGTDELKR
KQLMELAIINGTYRPMKSPN
PA
5'-CUACUCAUAU-3': CUACUCAUAU
Data type | Count |
13C chemical shifts | 386 |
15N chemical shifts | 144 |
1H chemical shifts | 1010 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GLD-1 KH-QUA2 bound to 5'-CUACUCAUAU-3' | 1 |
2 | RNA | 2 |
Entity 1, GLD-1 KH-QUA2 bound to 5'-CUACUCAUAU-3' 142 residues - 16075.667 Da.
1 | LEU | PRO | GLU | PRO | ALA | GLY | ASP | MET | ILE | SER | ||||
2 | ILE | THR | GLU | LYS | ILE | TYR | VAL | PRO | LYS | ASN | ||||
3 | GLU | TYR | PRO | ASP | TYR | ASN | PHE | VAL | GLY | ARG | ||||
4 | ILE | LEU | GLY | PRO | ARG | GLY | MET | THR | ALA | LYS | ||||
5 | GLN | LEU | GLU | GLN | ASP | THR | GLY | CYS | LYS | ILE | ||||
6 | MET | VAL | ARG | GLY | LYS | GLY | SER | MET | ARG | ASP | ||||
7 | LYS | SER | LYS | GLU | SER | ALA | HIS | ARG | GLY | LYS | ||||
8 | ALA | ASN | TRP | GLU | HIS | LEU | GLU | ASP | ASP | LEU | ||||
9 | HIS | VAL | LEU | VAL | GLN | CYS | GLU | ASP | THR | GLU | ||||
10 | ASN | ARG | VAL | HIS | ILE | LYS | LEU | GLN | ALA | ALA | ||||
11 | LEU | GLU | GLN | VAL | LYS | LYS | LEU | LEU | ILE | PRO | ||||
12 | ALA | PRO | GLU | GLY | THR | ASP | GLU | LEU | LYS | ARG | ||||
13 | LYS | GLN | LEU | MET | GLU | LEU | ALA | ILE | ILE | ASN | ||||
14 | GLY | THR | TYR | ARG | PRO | MET | LYS | SER | PRO | ASN | ||||
15 | PRO | ALA |
Entity 2, RNA 10 residues - 3082.892 Da.
1 | C | U | A | C | U | C | A | U | A | U |
15N: GLD-1 (aa 195-336), [U-15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; H2O 90%; D2O, [U-100% 2H], 10%
15N_13C: GLD-1 (aa 195-336), [U-13C; U-15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; H2O 90%; D2O, [U-100% 2H], 10%
15N_(D2O): GLD-1 (aa 195-336), [U-15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; D2O, [U-100% 2H], 100%
15N_13C_(D2O): GLD-1 (aa 195-336), [U-13C; U-15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; D2O, [U-100% 2H], 100%
15N_13C_(10): GLD-1 (aa 195-336), [U-10% 13C; U-100% 15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; H2O 90%; D2O, [U-100% 2H], 10%
15N_(Phage): GLD-1 (aa 195-336), [U-15N], 0.45 mM; 5'-CUACUCAUAU-3' 0.45 mM; sodium chloride 50 mM; sodium phosphate 20 mM; DTT 3 mM; Pf1 phage 14 mg/mL; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: ionic strength: 0.17 M; pH: 6.5; pressure: 1 atm; temperature: 303 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | 15N | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | 15N_13C_(D2O) | isotropic | sample_conditions_1 |
3D HNCA | 15N_13C | isotropic | sample_conditions_1 |
3D HN(CO)CA | 15N_13C | isotropic | sample_conditions_1 |
3D HNCO | 15N_13C | isotropic | sample_conditions_1 |
3D HN(CA)CO | 15N_13C | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | 15N_13C | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | 15N_13C | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | 15N | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | 15N_13C | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | 15N_(D2O) | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | 15N_(D2O) | isotropic | sample_conditions_1 |
2D F1f F2f 1H-1H NOESY | 15N_(D2O) | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | 15N | isotropic | sample_conditions_1 |
2D F2f 1H-1H NOESY | 15N_13C_(D2O) | isotropic | sample_conditions_1 |
3D F1f F2e 1H-1H NOESY | 15N_13C_(D2O) | isotropic | sample_conditions_1 |
Long-range 1H-15N HSQC | 15N | isotropic | sample_conditions_1 |
3D HNHA | 15N | isotropic | sample_conditions_1 |
2D 1H-15N IPAP HSQC | 15N_(Phage) | anisotropic | sample_conditions_1 |
2D 1H-13C HSQC | 15N_13C_(10) | isotropic | sample_conditions_1 |
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY v3.114, Goddard - chemical shift assignment
TOPSPIN v3.0, Bruker Biospin - collection
TALOS v+, Cornilescu, Delaglio and Bax - collection
ProcheckNMR v3.5.4, Laskowski and MacArthur - data analysis
ATNOSCANDID v2.1, Herrmann, Guntert and Wuthrich - peak picking
PALES v2.1, Zweckstetter, Hummer and Bax - data analysis
WhatIF v10.1.1, Vriend - data analysis
PDB | |
EMBL | CAB03417 |
GB | AAC46632 EFP08647 |
PRF | 2116296A |
REF | NP_492143 XP_003100676 |
SP | Q17339 |
AlphaFold | Q17339 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks