BMRB Entry 19641

Title:
3rCWP7D, variant of Imunoglobulin light-chain of lambda 3
Deposition date:
2013-11-27
Original release date:
2015-04-15
Authors:
Gil-Rodriguez, Paloma; Amero, Carlos
Citation:

Citation: Gil-Rodriguez, Paloma; Amero, Carlos. "(1)H, (15)N and (13)C resonance assignments for 3rC and 3rCWP: amyloidogenic variants of imunoglobulin lambda 3 light-chain"  Biomol NMR Assign. 9, 139-142 (2015).
PubMed: 24816896

Assembly members:

Assembly members:
3rCWP7D, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts107
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13rCWP7D1

Entities:

Entity 1, 3rCWP7D 107 residues - Formula weight is not available

1   SERTYRGLULEUTHRGLNASPPROSERVAL
2   SERVALSERPROGLYGLNTHRALASERILE
3   THRCYSSERGLYASPLYSLEUGLYASPLYS
4   TYRALATYRTRPTYRGLNGLNLYSPROGLY
5   GLNSERPROVALLEUVALILETYRGLNASP
6   SERLYSARGPROSERGLYILEPROGLUARG
7   PHESERGLYSERASNSERGLYASNTHRALA
8   THRLEUTHRILESERGLYTHRGLNALAMET
9   ASPGLUALAASPTYRTYRCYSGLNALAALA
10   ASPSERSERGLYALAVALVALPHEGLYGLY
11   GLYTHRLYSLEUTHRVALLEU

Samples:

sample_1: 3rCWP7D, [U-15N], 1 ± 0.3 mM; sodium phosphate 20 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_2: 3rCWP7D, [U-13C; U-15N], 1 ± 0.3 mM; sodium phosphate 20 mM; sodium chloride 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1

Software:

NMRPipe v7.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

BMRB 19671
PDB
GB AAR02898

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks