BMRB Entry 19635

Title:
Solution structure of TpsB4 N-terminal POTRA domain from Pseudomonas aeruginosa
Deposition date:
2013-11-25
Original release date:
2014-12-22
Authors:
Garnett, James; Matthews, Steve
Citation:

Citation: Garnett, James; Matthews, Steve. "Structure-function analysis reveals a dual role of the Pseudomonas aeruginosa Tps4 two-partner secretion system"  .

Assembly members:

Assembly members:
TpsB4, polymer, 95 residues, 10764.220 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46 LIC/Ek

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts83
1H chemical shifts607

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TpsB41

Entities:

Entity 1, TpsB4 95 residues - 10764.220 Da.

Residues 1-15 represent a non-native affinity tag. Residues 16-95 correspond to residues 42-121 of mature TpsB4.

1   METALAHISHISHISHISHISHISVALASP
2   ASPASPASPLYSMETGLYGLYPROTHRVAL
3   LEUVALLYSARGPHEGLNLEUSERGLYASN
4   ARGGLNPHEASPASPARGARGLEULEUALA
5   LEULEUHISASPLEUPROGLYGLNGLULEU
6   ASNLEUSERGLNLEUHISALAALAALAALA
7   ARGILEGLYASPPHETYRGLNGLULYSGLY
8   TYRVALLEUALAARGALAPHELEUPROALA
9   GLNGLUILEGLNASPGLYTHRVALARGILE
10   GLUVALLEUGLUGLY

Samples:

sample_1: TpsB4, [U-13C; U-15N], 1 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_2: TpsB4, [U-13C; U-15N], 1 mM; D2O, [U-100% 2H], 100%

sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

ARIA v2.1, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

DBJ BAK87931 BAP21481 BAP53040 BAQ42187 BAR69849
EMBL CAW29677 CCQ83911 CDH73159 CDH79418 CDI95309
GB AAG07928 ABJ13808 AEO77112 AFM66993 AGI83581
REF NP_253230 WP_003094678 WP_003112835 WP_003120951 WP_003122077

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks