BMRB Entry 19555

Title:
Solution structure of the C-terminally encoded peptide of the plant parasitic nematode Meloidogyne hapla - CEP11
Deposition date:
2013-10-14
Original release date:
2013-12-09
Authors:
Bobay, Benjamin; DiGennaro, Peter; McK. Bird, David
Citation:

Citation: Bobay, Benjamin; DiGennaro, Peter; Scholl, Elizabeth; Imin, Nijat; Djordjevic, Michael; McK. Bird, David. "Solution NMR studies of the plant peptide hormone CEP inform function"  FEBS Lett. 587, 3979-3985 (2013).
PubMed: 24211833

Assembly members:

Assembly members:
entity, polymer, 15 residues, 1522.660 Da.

Natural source:

Natural source:   Common Name: Northern root-knot nematode   Taxonomy ID: 6305   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meloidogyne hapla

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: synthetic   Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: AFRXTAPGHSXGVGH

Data sets:
Data typeCount
13C chemical shifts26
15N chemical shifts12
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CEP111

Entities:

Entity 1, CEP11 15 residues - 1522.660 Da.

1   ALAPHEARGHYPTHRALAPROGLYHISSER
2   HYPGLYVALGLYHIS

Samples:

sample_1: entity 4 mg/mL; DSS 10 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks