BMRB Entry 19485

Title:
Chemical Shift Assignments for SVIP (small VCP/p97-interacting protein)
Deposition date:
2013-09-10
Original release date:
2013-10-09
Authors:
Wu, Jiawen; Peng, Dungeng; Voehler, Markus; Sanders, Charles; Li, Jun
Citation:

Citation: Wu, Jiawen; Peng, Dungeng; Voehler, Markus; Sanders, Charles; Li, Jun. "Structure and expression of a novel compact myelin protein - small VCP-interacting protein (SVIP)."  Biochem. Biophys. Res. Commun. 440, 173-178 (2013).
PubMed: 24055875

Assembly members:

Assembly members:
SVIPM, polymer, 85 residues, 9475.68 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts280
15N chemical shifts67
1H chemical shifts477

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SVIP mutation1

Entities:

Entity 1, SVIP mutation 85 residues - 9475.68 Da.

Residues 78-85 represent a non-native affinity His-tag

1   METGLYLEUSERPHEPROSERPROGLYGLU
2   SERALAPROPROTHRPROASPLEUGLUGLU
3   LYSARGALALYSLEUALAGLUALAALAGLU
4   ARGARGGLNLYSGLUALAALASERARGGLY
5   ILELEUASPVALGLNSERVALGLNGLULYS
6   ARGLYSLYSLYSGLULYSILEGLULYSGLN
7   ILEALATHRSERGLYPROPROPROGLUGLY
8   GLYLEUARGTRPTHRVALSERLEUGLUHIS
9   HISHISHISHISHIS

Samples:

sample_1: SVIPM, [U-99% 13C; U-99% 15N], 0.4 – 0.6 mM; potassium phosphate 25 mM; EDTA 0.1 mM; sodium chloride 25 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GB NM_148893 AAM88869 EAW68312 EAW68313 EAW68314 EAW68315
EMBL CAH92580
REF NP_001126513 NP_001248626 NP_683691 XP_001173949 XP_003254381
SP Q5R6N0 Q8NHG7
AlphaFold Q5R6N0 Q8NHG7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks