BMRB Entry 19446

Title:
Single-stranded DNA binding protein from E. coli (SSB)
Deposition date:
2013-08-21
Original release date:
2013-09-19
Authors:
Shishmarev, Dmitry; Wang, Yao; Yagi, Hiromasa; Dixon, Nicholas; Su, Xun-Cheng; Yagi, Hiromasa
Citation:

Citation: Su, Xun-Cheng; Wang, Yao; Yagi, Hiromasa; Shishmarev, Dmitry; Mason, Claire; Smith, Paul; Vandevenne, Marylene; Dixon, Nicholas; Otting, Gottfried. "Bound or Free: Interaction of the C-Terminal Domain of Escherichia coli Single-Stranded DNA-Binding Protein (SSB) with the Tetrameric Core of SSB."  Biochemistry 53, 1925-1934 (2014).
PubMed: 24606314

Assembly members:

Assembly members:
SSB, polymer, 177 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Data sets:
Data typeCount
13C chemical shifts701
15N chemical shifts178
1H chemical shifts948

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Single-stranded DNA binding protein1

Entities:

Entity 1, Single-stranded DNA binding protein 177 residues - Formula weight is not available

1   ALASERARGGLYVALASNLYSVALILELEU
2   VALGLYASNLEUGLYGLNASPPROGLUVAL
3   ARGTYRMETPROASNGLYGLYALAVALALA
4   ASNILETHRLEUALATHRSERGLUSERTRP
5   ARGASPLYSALATHRGLYGLUMETLYSGLU
6   GLNTHRGLUTRPHISARGVALVALLEUPHE
7   GLYLYSLEUALAGLUVALALASERGLUTYR
8   LEUARGLYSGLYSERGLNVALTYRILEGLU
9   GLYGLNLEUARGTHRARGLYSTRPTHRASP
10   GLNSERGLYGLNASPARGTYRTHRTHRGLU
11   VALVALVALASNVALGLYGLYTHRMETGLN
12   METLEUGLYGLYARGGLNGLYGLYGLYALA
13   PROALAGLYGLYASNILEGLYGLYGLYGLN
14   PROGLNGLYGLYTRPGLYGLNPROGLNGLN
15   PROGLNGLYGLYASNGLNPHESERGLYGLY
16   ALAGLNSERARGPROGLNGLNSERALAPRO
17   ALAALAPROSERASNGLUPROPROMETASP
18   PHEASPASPASPILEPROPHE

Samples:

sample_1: SSB, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 3.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

Analysis v2.1.5, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB38464 BAE78061 BAG79877 BAI28321 BAI33498
EMBL CAP78525 CAQ34408 CAQ91583 CAR01039 CAR05708
GB AAA24649 AAC43153 AAC77029 AAG59257 AAN45567
PIR E86099
REF NP_313068 NP_418483 NP_709860 WP_000157064 WP_000168295
SP P0AGE0 P0AGE1 P0AGE2 P0AGE3
AlphaFold P0AGE0 P0AGE1 P0AGE2 P0AGE3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks