BMRB Entry 19392

Title:
Human Bcl10 CARD
Deposition date:
2013-07-26
Original release date:
2013-10-14
Authors:
Zheng, Chao; Bracken, Clay; Wu, Hao
Citation:

Citation: Qiao, Qi; Yang, Chenghua; Zheng, Chao; Fontan, Lorena; David, Liron; Yu, Xiong; Bracken, Clay; Rosen, Monica; Melnick, Ari; Egelman, Edward; Wu, Hao. "Structural Architecture of the CARMA1/Bcl10/MALT1 Signalosome: Nucleation-Induced Filamentous Assembly"  Mol. Cell 51, 766-779 (2013).
PubMed: 24074955

Assembly members:

Assembly members:
Bcl10, polymer, 118 residues, 12642.762 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts466
15N chemical shifts116
1H chemical shifts777

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bcl10_11
2Bcl10_21
3Bcl10_31
4Bcl10_41
5Bcl10_51
6Bcl10_61
7Bcl10_71
8Bcl10_81
9Bcl10_91
10Bcl10_101
11Bcl10_111
12Bcl10_121
13Bcl10_131
14Bcl10_141
15Bcl10_151
16Bcl10_161
17Bcl10_171
18Bcl10_181
19Bcl10_191
20Bcl10_201

Entities:

Entity 1, Bcl10_1 118 residues - 12642.762 Da.

1   GLYSERHISMETGLUPROTHRALAPROSER
2   LEUTHRGLUGLUASPLEUTHRGLUVALLYS
3   LYSASPALALEUGLUASNLEUARGVALTYR
4   LEUCYSGLULYSILEILEALAGLUARGHIS
5   PHEASPHISLEUARGALALYSLYSILELEU
6   SERARGGLUASPTHRARGGLUILESERCYS
7   ARGTHRSERSERARGLYSARGALAGLYLYS
8   LEULEUASPTYRLEUGLNGLUASNPROLYS
9   GLYLEUASPTHRLEUVALGLUSERILEARG
10   ARGGLULYSTHRGLNASNPHELEUILEGLN
11   LYSILETHRASPGLUVALLEULYSLEUARG
12   ASNILELYSLEUGLUHISLEULYS

Samples:

sample_1: Bcl10, [U-13C; U-15N], 0.3 – 0.4 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAA88822 BAF84035 BAI45655
EMBL CAA06955
GB AAC99767 AAD15800 AAD16428 AAD24918 AAD32597
REF NP_001071496 NP_001096683 NP_001248144 NP_003912 NP_112618
SP O95999 Q9QYN5
TPG DAA31335
AlphaFold O95999 Q9QYN5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks