BMRB Entry 19307

Title:
Magnesium bound form of UVI31+ from C. reinhardtii
Deposition date:
2013-06-18
Original release date:
2014-02-13
Authors:
Singh, Himanshu; Chary, Kandala; Verma, Deepshikha
Citation:

Citation: Singh, Himanshu; Rao, B.; Chary, Kandala. "(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23979961

Assembly members:

Assembly members:
UVI31+Mg, polymer, 123 residues, Formula weight is not available
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Chlamydomonas reinhardtii   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET and pQE-30

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts106
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UVI31+Mg1
2Mg2

Entities:

Entity 1, UVI31+Mg 123 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERHISVALILESERSERILEALASER
3   ARGGLYSERMETALAGLUHISGLNLEUGLY
4   PROILEALAGLYALAILELYSSERLYSVAL
5   GLUALAALALEUSERPROTHRHISPHELYS
6   LEUILEASNASPSERHISLYSHISALAGLY
7   HISTYRALAARGASPGLYSERTHRALASER
8   ASPALAGLYGLUTHRHISPHEARGLEUGLU
9   VALTHRSERASPALAPHELYSGLYLEUTHR
10   LEUVALLYSARGHISGLNLEUILETYRGLY
11   LEULEUSERASPGLUPHELYSALAGLYLEU
12   HISALALEUSERMETTHRTHRLYSTHRPRO
13   ALAGLUGLN

Entity 2, Mg - Mg - 24.305 Da.

1   MG

Samples:

sample_1: UVI31+Mg, [U-99% 13C; U-99% 15N], 0.6 mM; sodium phosphate 50 mM; NaCl 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16864 18567 18969 19308
PDB
GB EDO96758
REF XP_001702905

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks