BMRB Entry 19274

Title:
Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC
Deposition date:
2013-05-28
Original release date:
2014-12-22
Authors:
Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven
Citation:

Citation: Liburd, Janine; Miller, Emily; Langelaan, David; Chitayat, Seth; Crawley, Scott; Cote, Graham; Smith, Steven. "Solution Structure of the Dictyostelium discodieum Myosin Light Chain, MlcC"  .

Assembly members:

Assembly members:
entity, polymer, 77 residues, 8918.021 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts320
15N chemical shifts81
1H chemical shifts509

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dictyostelium discodieum Myosin Light Chain, MlcC1

Entities:

Entity 1, Dictyostelium discodieum Myosin Light Chain, MlcC 77 residues - 8918.021 Da.

1   GLYSERHISMETASNHISILEASNTHRLYS
2   ALAGLNVALILEGLUALAPHELYSVALPHE
3   ASPARGASPGLYASNGLYTYRVALTHRVAL
4   ASPTYRLEUARGLYSVALLEUASNGLULEU
5   GLYASPMETMETPROALAASPGLUILEGLU
6   GLUMETILETYRGLUALAASPPROGLNASN
7   SERGLYTYRVALGLNTYRGLUTHRPHEVAL
8   GLYMETLEUPHELEUTRPASP

Samples:

sample_1: entity 1 mM; HEPES 10 mM; sodium chloride 50 mM; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

GB EAL62662
REF XP_636165

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks