BMRB Entry 19270

Title:
Backbone assignment of the Sigma-1 receptor chaperone domain
Deposition date:
2013-05-27
Original release date:
2014-02-13
Authors:
Ortega-Roldan, Jose L.; Schnell, Jason
Citation:

Citation: Ortega-Roldan, Jose Luis; Ossa, Felipe; Schnell, Jason. "Characterization of the human sigma-1 receptor chaperone domain structure and binding immunoglobulin protein (BiP) interactions."  J. Biol. Chem. 288, 21448-21457 (2013).
PubMed: 23760505

Assembly members:

Assembly members:
S1ch, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCold-I

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts105
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S1ch1

Entities:

Entity 1, S1ch 129 residues - Formula weight is not available

1   METASNHISLYSVALHISHISHISHISHIS
2   HISILEGLUGLYARGHISMETGLYSERARG
3   GLYHISSERGLYARGTYRTRPALAGLUILE
4   SERASPTHRILEILESERGLYTHRPHEHIS
5   GLNTRPARGGLUGLYTHRTHRLYSSERGLU
6   VALPHETYRPROGLYGLUTHRVALVALHIS
7   GLYPROGLYGLUALATHRALAVALGLUTRP
8   GLYPROASNTHRTRPMETVALGLUTYRGLY
9   ARGGLYVALILEPROSERTHRLEUALAPHE
10   ALALEUALAASPTHRVALPHESERTHRGLN
11   ASPPHELEUTHRLEUPHETYRTHRLEUARG
12   SERTYRALAARGGLYLEUARGLEUGLULEU
13   THRTHRTYRLEUPHEGLYGLNASPPRO

Samples:

sample_1: S1ch, [U-100% 13C; U-100% 15N; U-80% 2H], 0.27 mM; S1ch 95%; S1ch 5%

sample_2: S1ch, [U-100% 13C; U-100% 15N], 0.27 mM; S1ch 95%; S1ch 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

EMBL CAG33356
GB AAB50402 AAB51238 AAC04507 AAH04899 ABM83807
REF NP_001244806 NP_001269135 NP_001269136 NP_005857 XP_002743083
SP Q99720
AlphaFold Q99720

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks