BMRB Entry 19269

Title:
1H and 13C chemical shift assignment of the N-terminal intrinsically disordered domain of the oncoportein E7 from the Human Papilloma Virus
Deposition date:
2013-05-27
Original release date:
2014-02-13
Authors:
Gallo, Mariana; Noval, Maria; Chemes, Lucia; de Prat Gay, Gonzalo
Citation:

Citation: Noval, Maria; Gallo, Mariana; Perrone, Sebastian; Salvay, Andres; Chemes, Lucia; de Prat-Gay, Gonzalo. "Conformational dissection of a viral intrinsically disordered domain involved in cellular transformation."  PLoS ONE 8, e72760-e72760 (2013).
PubMed: 24086265

Assembly members:

Assembly members:
E7N, polymer, 40 residues, 4678 Da.

Natural source:

Natural source:   Common Name: Human papillomavirus   Taxonomy ID: 10566   Superkingdom: Viruses   Kingdom: not available   Genus/species: Papillomavirus Human Papillomavirus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E7N: MHGDTPTLHEYMLDLQPETT DLYCYEQLNDSSEEEDEIDG

Data sets:
Data typeCount
13C chemical shifts133
1H chemical shifts263

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E7N1

Entities:

Entity 1, E7N 40 residues - 4678 Da.

1   METHISGLYASPTHRPROTHRLEUHISGLU
2   TYRMETLEUASPLEUGLNPROGLUTHRTHR
3   ASPLEUTYRCYSTYRGLUGLNLEUASNASP
4   SERSERGLUGLUGLUASPGLUILEASPGLY

Samples:

sample_1: E7N 3 mM; TCEP 10 mM; H2O 90%; D2O 10%

sample_2: E7N 3 mM; TCEP 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.21 M; pH: 5.00; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC TOCSYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19442
DBJ BAA00633 BAN15982 BAN15983 BAN15984 BAN15985
EMBL CAB45105 CAB45107 CAB45109 CAB45111 CAB45113
GB AAA46940 AAB18962 AAB18963 AAB18964 AAB70737
PRF 2002324A
REF NP_041326 WP_053289714
SP P03129
AlphaFold P03129