BMRB Entry 19247

Title:
Backbone (1H,15N, CA, CB) assignments of Populus nigra Plastocyanin (Zn)
Deposition date:
2013-05-17
Original release date:
2013-06-04
Authors:
Guan, Jia-Ying; Ubbink, Marcellus
Citation:

Citation: Guan, Jia-Ying; Foester, Johannes; Drijfhout, Jan Wouter; Timmer, Monika; Blok, Anneloes; Ullmann, Matthias; Ubbink, Marcellus. "Dynamics between plastocyanin and tetralysine peptides"  .

Assembly members:

Assembly members:
plastocyanin, polymer, 101 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Lombardy poplar   Taxonomy ID: 3691   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Populus nigra

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts226
15N chemical shifts114
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1plastocyanin1
2zinc2

Entities:

Entity 1, plastocyanin 101 residues - Formula weight is not available

1.The first two residues in the sequence (MG) are not included in the assignment data. The residue numbers in the assignment correspond to the sequence starting from the third residue (I). 2.N-terminal methionine was partially processed and therefore some residues near N-terminus in space have double resonances.

1   METGLYILEASPVALLEULEUGLYALAASP
2   ASPGLYSERLEUALAPHEVALPROSERGLU
3   PHESERILESERPROGLYGLULYSILEVAL
4   PHELYSASNASNALAGLYPHEPROHISASN
5   ILEVALPHEASPGLUASPSERILEPROSER
6   GLYVALASPALASERLYSILESERMETSER
7   GLUGLUASPLEULEUASNALALYSGLYGLU
8   THRPHEGLUVALALALEUSERASNLYSGLY
9   GLUTYRSERPHETYRCYSSERPROHISGLN
10   GLYALAGLYMETVALGLYLYSVALTHRVAL
11   ASN

Entity 2, zinc - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: plastocyanin, [U-13C; U-15N], 0.25 ± 0.01 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
BMRB 4019
EMBL CAA90564
GB ABK96770 EEE94750
REF XP_002307754 XP_011003094
SP P00299
AlphaFold P00299

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks