BMRB Entry 19008

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19008

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Chemical Shift Assignments for SinR from Bacillus subtilis
Deposition date:
2013-02-05
Original release date:
2013-03-13
Authors:
Stowe, Sean; Olson, Andrew; Cavanagh, John
Citation:

Citation: Stowe, Sean; Olson, Andrew; Losick, Richard; Cavanagh, John. "Chemical shift assignments and secondary structure prediction of the master biofilm regulator, SinR, from Bacillus subtilis."  Biomol. NMR Assignments 8, 155-158 (2014).
PubMed: 23475644

Assembly members:

Assembly members:
SinR_FL, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SinR_FL: MIGQRIKQYRKEKGYSLSEL AEKAGVAKSYLSSIERNLQT NPSIQFLEKVSAVLDVSVHT LLDEKHETEYDGQLDSEWEK LVRDAMTSGVSKKQFREFLD YQKWRKSQKEE

Data sets:
Data typeCount
13C chemical shifts489
15N chemical shifts117
1H chemical shifts602

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SinR_FL_11
2SinR_FL_21
3SinR_FL_31
4SinR_FL_41

Entities:

Entity 1, SinR_FL_1 111 residues - Formula weight is not available

1   METILEGLYGLNARGILELYSGLNTYRARG
2   LYSGLULYSGLYTYRSERLEUSERGLULEU
3   ALAGLULYSALAGLYVALALALYSSERTYR
4   LEUSERSERILEGLUARGASNLEUGLNTHR
5   ASNPROSERILEGLNPHELEUGLULYSVAL
6   SERALAVALLEUASPVALSERVALHISTHR
7   LEULEUASPGLULYSHISGLUTHRGLUTYR
8   ASPGLYGLNLEUASPSERGLUTRPGLULYS
9   LEUVALARGASPALAMETTHRSERGLYVAL
10   SERLYSLYSGLNPHEARGGLUPHELEUASP
11   TYRGLNLYSTRPARGLYSSERGLNLYSGLU
12   GLU

Samples:

sample_1: SinR_FL, [U-98% 13C; U-98% 15N], 450 uM; MES 20 mM; sodium chloride 200 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_2: SinR_FL, [U-98% 13C; U-98% 15N], 450 uM; MES 20 mM; sodium chloride 200 mM; sodium azide 0.02%; D2O 100%

sample_conditions_1: ionic strength: 220 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis, peak picking

PINE, Eghbalnia, Wang, Bahrami, Assadi, and Markley - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA12542 BAI85980 BAM52946 BAM58520 GAK78309
EMBL CAB14392 CBI43490 CCF05840 CCG50467 CCP22253
GB AAA22757 ABS74654 ADM38475 ADP33055 ADV93205
REF NP_390341 WP_003226345 WP_003230185 WP_010789193 WP_013352861
SP P06533
AlphaFold P06533

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks