BMRB Entry 18969

Title:
1H, 13C and 15N resonance assignments of S55A mutant of UVI31+ from Chlamydomonas reinhardtii
Deposition date:
2013-01-22
Original release date:
2014-02-14
Authors:
Singh, Himanshu; Chary, Kandala
Citation:

Citation: Singh, Himanshu; Rao, B.; Chary, Kandala. "(1)H, (13)C and (15)N NMR assignments of a mutant of UV inducible transcript (S55A-UVI31+) from Chlamydomonas reinhardtii."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23979961

Assembly members:

Assembly members:
S55A_of_UVI31+, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chlamydomonas reinhardtii   Taxonomy ID: 3055   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Chlamydomonas reinhardtii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: chlamydomonas reinhardtii   Vector: pET and pQE-30

Data sets:
Data typeCount
13C chemical shifts288
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S55A mutant of UVI31+1

Entities:

Entity 1, S55A mutant of UVI31+ 123 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERHISVALILESERSERILEALASER
3   ARGGLYSERMETALAGLUHISGLNLEUGLY
4   PROILEALAGLYALAILELYSSERLYSVAL
5   GLUALAALALEUSERPROTHRHISPHELYS
6   LEUILEASNASPSERHISLYSHISALAGLY
7   HISTYRALAARGASPGLYSERTHRALASER
8   ASPALAGLYGLUTHRHISPHEARGLEUGLU
9   VALTHRSERASPALAPHELYSGLYLEUTHR
10   LEUVALLYSARGHISGLNLEUILETYRGLY
11   LEULEUSERASPGLUPHELYSALAGLYLEU
12   HISALALEUALAMETTHRTHRLYSTHRPRO
13   ALAGLUGLN

Samples:

sample_1: S55A of UVI31+, [U-100% 13C; U-100% 15N], 0.8 ± 0.03 mM; sodium phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

FELIX v2002, accelrys Inc. - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18567 19307 19308
PDB
GB EDO96758
REF XP_001702905

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks