BMRB Entry 18961

Name: NMR assignments of a hypothetical pseudo-knotted protein HP0242 from H. pylori
Published: 2014-02-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18961

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignments of a hypothetical pseudo-knotted protein HP0242 from H. pylori

Deposition date:

2013-01-18

Original release date:

2014-02-14

Authors:

Chien, Chih-Ta Henry; Wang, Lian-Wei; Liu, Yu-Nan; Lyu, Ping-Chiang; Hsu, Shang-Te Danny

Citation:

Citation: Chien, Chih-Ta Henry; Wang, Liang-Wei; Liu, Yu-Nan; Hsu, Ban-Dar; Lyu, Ping-Chiang; Hsu, Shang-Te Danny. "NMR assignments of a hypothetical pseudo-knotted protein HP0242 from Helicobacter pylori." Biomol. NMR Assignments 8, 287-289 (2013).
PubMed: 23824732

Assembly members:

Assembly members:
HP0242, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-32a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HP0242: MRDYSELEIFEGNPLDKWND IIFHASKKLSKKELERLLEL LALLETFIEKEDLEEKFESF AKALRIDEELQQKIESRKTD IVIQSMANILSGNE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	419
15N chemical shifts	91
1H chemical shifts	639

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP0242	1

Entities:

Entity 1, HP0242 94 residues - Formula weight is not available

1

MET

ARG

ASP

TYR

SER

GLU

LEU

GLU

ILE

PHE

2

GLU

GLY

ASN

PRO

LEU

ASP

LYS

TRP

ASN

ASP

3

ILE

PHE

HIS

ALA

SER

LYS

LEU

SER

4

LYS

GLU

LEU

GLU

ARG

LEU

GLU

LEU

5

LEU

ALA

LEU

GLU

THR

PHE

ILE

GLU

LYS

6

GLU

ASP

LEU

GLU

LYS

PHE

GLU

SER

PHE

7

ALA

LYS

ALA

LEU

ARG

ILE

ASP

GLU

LEU

8

GLN

LYS

ILE

GLU

SER

ARG

LYS

THR

ASP

9

ILE

VAL

ILE

GLN

SER

MET

ALA

ASN

ILE

LEU

10

SER

GLY

ASN

GLU

Samples:

sample_1: HP0242, [U-100% 13C; U-100% 15N], 818 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks