BMRB Entry 18909

Title:
Solution NMR structure of the SANT domain of human DNAJC2; Notrheast structural genomics consortium target HR8254a
Deposition date:
2012-12-18
Original release date:
2013-02-08
Authors:
Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl
Citation:

Citation: Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl. "NMR solution structure of the SANT domain of human DnaJC2."  .

Assembly members:

Assembly members:
entity, polymer, 73 residues, 8308.837 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28-MHL

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts332
15N chemical shifts74
1H chemical shifts541

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hs005071

Entities:

Entity 1, hs00507 73 residues - 8308.837 Da.

1   GLNGLYPHETHRPROTRPTHRTHRGLUGLU
2   GLNLYSLEULEUGLUGLNALALEULYSTHR
3   TYRPROVALASNTHRPROGLUARGTRPLYS
4   LYSILEALAGLUALAVALPROGLYARGTHR
5   LYSLYSASPCYSMETLYSARGTYRLYSGLU
6   LEUVALGLUMETVALLYSALALYSLYSALA
7   ALAGLNGLUGLNVALLEUASNALASERARG
8   ALALYSLYS

Samples:

sample_1: hs00507, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; benzamidine 10 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

FMC, Lemak,Steren,Llinas, Arrowsmith - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

PSVS, Bhattacharya and Montelione - validation

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAE00600 BAK62178
EMBL CAA66913
GB AAH00859 AAI14888 AAI25058 AAI39752 AAI60045
REF NP_001068805 NP_001123359 NP_001247727 NP_001267346 NP_001270315
SP Q1RMH9 Q4R8H2 Q99543
TPG DAA30686
AlphaFold Q99543 Q1RMH9 Q4R8H2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks