BMRB Entry 18840

Title:
The ZZ domain of cytoplasmic polyadenylation element binding protein 1 (CPEB1)
Deposition date:
2012-11-14
Original release date:
2013-04-01
Authors:
Lee, Brian; Merkel, Daniel; Wells, Sarah; Hilburn, Bryce; Elazzouzi, Fatima; Perez-Alvarado, Gabriela
Citation:

Citation: Merkel, Daniel; Wells, Sarah; Hilburn, Bryce; Elazzouzi, Fatima; Perez-Alvarado, Gabriela; Lee, Brian. "The C-Terminal Region of Cytoplasmic Polyadenylation Element Binding Protein Is a ZZ Domain with Potential for Protein-Protein Interactions."  J. Mol. Biol. 425, 2015-2026 (2013).
PubMed: 23500490

Assembly members:

Assembly members:
CPEB1-ZZ_G-P-504-566, polymer, 65 residues, 7768.881 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMal

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CPEB1-ZZ_G-P-504-566: GPVQIDPYLEDSLCHICSSQ PGPFFCRDQVCFKYFCRSCW HWRHSMEGLRHHSPLMRNQK NRDSS

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts76
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CPEB1-ZZ_G-P-504-5661
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, CPEB1-ZZ_G-P-504-566 65 residues - 7768.881 Da.

Residues Gly502 and Pro503 are non-native remnants of HRV 3C digest from MBP fusion

1   GLYPROVALGLNILEASPPROTYRLEUGLU
2   ASPSERLEUCYSHISILECYSSERSERGLN
3   PROGLYPROPHEPHECYSARGASPGLNVAL
4   CYSPHELYSTYRPHECYSARGSERCYSTRP
5   HISTRPARGHISSERMETGLUGLYLEUARG
6   HISHISSERPROLEUMETARGASNGLNLYS
7   ASNARGASPSERSER

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

ZZ-504: CPEB1-ZZ G-P-504-566, [U-100% 13C; U-100% 15N], 0.3 mM; TRIS 20 mM; sodium chloride 200 mM; DTT 1 mM; H2O 90%; D2O 10%

ZZ-498: CPEB1-ZZ T498S-566, [U-100% 13C; U-100% 15N], 0.3 mM; potassium phosphate 50 mM; potassium chloride 200 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCZZ-504isotropicsample_conditions_1
2D 1H-15N HSQCZZ-498isotropicsample_conditions_1
2D 1H-1H NOESYZZ-504isotropicsample_conditions_1
2D 1H-1H NOESYZZ-498isotropicsample_conditions_1
2D 1H-13C ct-HSQC aliphaticZZ-504isotropicsample_conditions_1
2D 1H-13C ct-HSQC aliphaticZZ-498isotropicsample_conditions_1
2D 1H-13C HMQC aromaticZZ-504isotropicsample_conditions_1
2D 1H-13C HMQC aromaticZZ-498isotropicsample_conditions_1
2D 1H-15N HMQC 2JNHZZ-498isotropicsample_conditions_1
2D (HB)CB(CGCD)HDZZ-504isotropicsample_conditions_1
3D HNCOZZ-504isotropicsample_conditions_1
3D HNCOZZ-498isotropicsample_conditions_1
3D HNCAZZ-504isotropicsample_conditions_1
3D HNCAZZ-498isotropicsample_conditions_1
3D HNCACBZZ-504isotropicsample_conditions_1
3D CBCA(CO)NHZZ-504isotropicsample_conditions_1
3D CBCA(CO)NHZZ-498isotropicsample_conditions_1
3D HCCH-COSYZZ-504isotropicsample_conditions_1
3D HCCH-COSYZZ-498isotropicsample_conditions_1
3D 1H-15N/13C NOESYZZ-504isotropicsample_conditions_1
3D 1H-15N/13C NOESYZZ-498isotropicsample_conditions_1
3D HCCH-TOCSYZZ-504isotropicsample_conditions_1
3D C(CO)NHZZ-504isotropicsample_conditions_1

Software:

AMBER v10, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v1.0.5, Guntert, Mumenthaler and Wuthrich - refinement

TALOS_plus, Shen, Delaglio, Cornilescu and Bax - chemical shift calculation

Molmol, Koradi, Billeter and Wuthrich - data analysis

ProcheckNMR, Laskowski and MacArthur - data analysis

NMRView v8.0.3, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

UNP Q9BZB8
BMRB 19771
PDB
DBJ BAB14496 BAB33089 BAC37017 BAG63194 BAH11723
EMBL CAA69588 CAH92427
GB AAH35348 AAH50629 AAI25477 AAK01239 AAK01240
REF NP_001073001 NP_001073002 NP_001073003 NP_001099746 NP_001126432
SP P0C279 P70166 Q5R733 Q9BZB8
TPG DAA17635
AlphaFold Q9H8V5 P0C279 P70166 Q5R733 Q9BZB8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks