BMRB Entry 18718

Title:
Alpha4 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift
Deposition date:
2012-09-17
Original release date:
2013-07-25
Authors:
Bonet, Roman; Campbell, Iain
Citation:

Citation: Bonet, Roman; Vakonakis, Ioannis; Campbell, Iain. "Characterization of 14-3-3- Interactions with integrin tails."  J. Mol. Biol. 425, 3060-3072 (2013).
PubMed: 23763993

Assembly members:

Assembly members:
alpha_4_integrin, polymer, 37 residues, 4322.7 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16-b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha_4_integrin: GPLGSKAGFFKRQYKSILQE ENRRDSWSYINSKSNDD

Data sets:
Data typeCount
15N chemical shifts36
1H chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha 4 integrin tail1

Entities:

Entity 1, alpha 4 integrin tail 37 residues - 4322.7 Da.

This is the cytoplasmic tail of the alpha 4 integrin tail. The first five residues (GPLGS) are a cloning artifact

1   GLYPROLEUGLYSERLYSALAGLYPHEPHE
2   LYSARGGLNTYRLYSSERILELEUGLNGLU
3   GLUASNARGARGASPSERTRPSERTYRILE
4   ASNSERLYSSERASNASPASP

Samples:

sample: alpha 4 integrin, [U-100% 13C; U-100% 15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DSS 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsampleisotropicsample_conditions_1
3D HNCACBsampleisotropicsample_conditions_1
2D 1H-15N HSQCsampleisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

UNP P13612
PIR S06046
GB L12002 AAB25486 AAB59613 AAH80190 AAI46278 AAI56713
DBJ BAD92122 BAI45328
EMBL CAA34852 CAD53328 CAD53329
REF NP_000876 XP_001100929 XP_002763652 XP_002812692 XP_003253848
SP P13612
AlphaFold Q7Z4L6 P13612

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks